DNA Ligands that Bind Tightly and Selectively to Cellobiose

Cell surface oligosaccharides have been shown to play essential biological roles in such diverse biological phenomena as cellular adhesion, molecular recognition, and inflammatory response. The development of high-affinity ligands capable of selectively recognizing a variety of small motifs in diffe...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-05, Vol.95 (10), p.5462-5467
Main Authors: Yang, Qing, Goldstein, Irwin J., Mei, Houng-Yau, Engelke, David R.
Format: Article
Language:English
Subjects:
Antigens
Biological Sciences
Carbohydrate Conformation
Carbohydrate Sequence
Cellobiose - metabolism
Cellular biology
Cellulose - metabolism
Deoxyribonucleic acid
Disaccharides
DNA
DNA - metabolism
Elution
Gene Library
Kinetics
Libraries
Ligands
Molecular Sequence Data
Molecules
Nucleotide aptamers
Nucleotides
Oligonucleotides
Sequence Analysis, DNA
Sugar
Sugars
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell surface oligosaccharides have been shown to play essential biological roles in such diverse biological phenomena as cellular adhesion, molecular recognition, and inflammatory response. The development of high-affinity ligands capable of selectively recognizing a variety of small motifs in different oligosaccharides would be of significant interest as experimental and diagnostic tools. As a step toward this goal we have developed DNA ligands that recognize the disaccharide cellobiose, whether in soluble form or as the repeating unit of the polymer, cellulose. These DNA ``aptamers'' bind with high selectivity to cellobiose with little or no affinity for the related disaccharides lactose, maltose, and gentiobiose. Thus, the DNA ligands can discriminate sugar epimers, anomers, and disaccharide linkages.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.10.5462