Electromagnetic Field-induced Stimulation of Bruton's Tyrosine Kinase

Here we present evidence that exposure of DT40 lymphoma B-cells to low energy electromagnetic fields (EMF) results in activation of phospholipase C-γ 2 (PLC-γ2), leading to increased inositol phospholipid turnover. PLC-γ2 activation in EMF-stimulated cells is mediated by stimulation of the Bruton�...

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Published in:The Journal of biological chemistry 1998-05, Vol.273 (20), p.12397-12401
Main Authors: Kristupaitis, Daiva, Dibirdik, Ilker, Vassilev, Alexei, Mahajan, Sandeep, Kurosaki, Tomohiro, Chu, Alice, Tuel-Ahlgren, Lisa, Tuong, Dong, Pond, David, Luben, Richard, Uckun, Fatih M.
Format: Article
Language:English
Subjects:
Agammaglobulinaemia Tyrosine Kinase
Animals
Chickens
Electromagnetic Fields
Enzyme Activation
Isoenzymes - metabolism
Lymphoma, B-Cell - enzymology
Lymphoma, B-Cell - pathology
Phospholipase C gamma
Protein-Tyrosine Kinases - metabolism
Tumor Cells, Cultured
Type C Phospholipases - metabolism
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cited_by cdi_FETCH-LOGICAL-c420t-c61ed94866afe474fc632680d31452db3c5a13d68bf30450f6a61650237d556e3
cites cdi_FETCH-LOGICAL-c420t-c61ed94866afe474fc632680d31452db3c5a13d68bf30450f6a61650237d556e3
container_end_page 12401
container_issue 20
container_start_page 12397
container_title The Journal of biological chemistry
container_volume 273
creator Kristupaitis, Daiva
Dibirdik, Ilker
Vassilev, Alexei
Mahajan, Sandeep
Kurosaki, Tomohiro
Chu, Alice
Tuel-Ahlgren, Lisa
Tuong, Dong
Pond, David
Luben, Richard
Uckun, Fatih M.
description Here we present evidence that exposure of DT40 lymphoma B-cells to low energy electromagnetic fields (EMF) results in activation of phospholipase C-γ 2 (PLC-γ2), leading to increased inositol phospholipid turnover. PLC-γ2 activation in EMF-stimulated cells is mediated by stimulation of the Bruton's tyrosine kinase (BTK), a member of the Src-related TEC family of protein tyrosine kinases, which acts downstream of LYN kinase and upstream of PLC-γ2. B-cells rendered BTK-deficient by targeted disruption of thebtk gene did not show enhanced PLC-γ2 activation in response to EMF exposure. Introduction of the wild-type (but not a kinase domain mutant) human btk gene into BTK-deficient B-cells restored their EMF responsiveness. Thus, BTK exerts a pivotal and mandatory function in initiation of EMF-induced signaling cascades in B-cells.
doi_str_mv 10.1074/jbc.273.20.12397
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source ScienceDirect Journals
subjects Agammaglobulinaemia Tyrosine Kinase
Animals
Chickens
Electromagnetic Fields
Enzyme Activation
Isoenzymes - metabolism
Lymphoma, B-Cell - enzymology
Lymphoma, B-Cell - pathology
Phospholipase C gamma
Protein-Tyrosine Kinases - metabolism
Tumor Cells, Cultured
Type C Phospholipases - metabolism
title Electromagnetic Field-induced Stimulation of Bruton's Tyrosine Kinase
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