Cloning of a breakpoint cluster region on chromosome 14 in uterine leiomyoma

A recurrent reciprocal chromosomal translocation, t(12;14)(q15;q24) is frequently observed in uterine leiomyoma. Chromosome 12 breakpoints have been shown to occur in a region of approximately 150 kb that contains the gene for a high mobility group protein (HMGI-C). The breakpoint region on chromoso...

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Bibliographic Details
Published in:Cancer letters 1998-04, Vol.126 (2), p.119-126
Main Authors: Bhugra, Bindu, Smolarek, Teresa A, Lynch, Roy A, Meloni, Aurelia M, Sandberg, Avery A, Deaven, Larry, Menon, Anil G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Breakpoint cluster region
Chromosome 14
Chromosomes, Artificial, Yeast - genetics
Chromosomes, Human, Pair 12 - genetics
Chromosomes, Human, Pair 14 - genetics
Cloning, Molecular
DNA, Neoplasm - genetics
Female
Female genital diseases
Genetic Markers
Gynecology. Andrology. Obstetrics
Humans
Karyotyping
Leiomyoma - genetics
Medical sciences
Polymerase Chain Reaction
Translocation, Genetic - genetics
Tumors
Uterine leiomyoma
Uterine Neoplasms - genetics
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Summary:A recurrent reciprocal chromosomal translocation, t(12;14)(q15;q24) is frequently observed in uterine leiomyoma. Chromosome 12 breakpoints have been shown to occur in a region of approximately 150 kb that contains the gene for a high mobility group protein (HMGI-C). The breakpoint region on chromosome 14 has not been precisely defined. We have generated a contig of overlapping yeast artificial chromosome (YAC) clones approximately 3 Mb in size. Fluorescence in situ hybridization (FISH) analysis showed that this contig spanned the t(12;14) breakpoints in three uterine leiomyomas and that the breakpoints in these tumors occurred within a 1 Mb region. A 30 kb cosmid spanning one of the breakpoints was isolated to set the stage for identifying regions on chromosome 14 that may cause this region to be a preferential site for chromosomal translocation.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(97)00478-3