Consecutive Enzyme Cascades: Complement Activation at the Cell Surface Triggers Increased Tissue Factor Activity

Complement activation at the cell surface initiates cell damage through a series of reactions occurring at the cell membrane and. after assembly of the terminal membrane attack complex, produces leakage of cytoplasmic contents from the cell. It has been documented that chemical or physical damage to...

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Bibliographic Details
Published in:Blood 1990-07, Vol.76 (2), p.361-367
Main Authors: Carson, Steven D., Johnson, Donald R.
Format: Article
Language:English
Subjects:
Antibodies - immunology
Biological and medical sciences
Cell Line
Cell Membrane - immunology
Cell Membrane - physiology
Chromium - metabolism
Complement
Complement Activation - physiology
Enzyme-Linked Immunosorbent Assay
Factor VIIa - metabolism
Factor X - metabolism
Fibroblasts - immunology
Fibroblasts - physiology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Kinetics
Molecular immunology
T-Lymphocytes - immunology
Thromboplastin - physiology
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Summary:Complement activation at the cell surface initiates cell damage through a series of reactions occurring at the cell membrane and. after assembly of the terminal membrane attack complex, produces leakage of cytoplasmic contents from the cell. It has been documented that chemical or physical damage to cell membranes can cause a rapid increase in the expression of tissue factor procoagulant activity. In this study, antibody-mediated complement activation at the cell surface resulted in increased tissue factor activity, which correlated with cytolysis, as measured by 51 -chromium release. Therefore, complement fixation on the cell surface can have a direct and immediate stimulatory effect on the coagulation cascade at the point of its initiation, with formation of a fibrin clot requiring only three consecutive proteolytic reactions after immunologically mediated cell damage.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V76.2.361.361