Galactosylated glycan expression and macrophage sensitivity of lewis lung tumor cells with different metastatic phenotype

Biochemical and cytochemical analysis of Lewis lung tumor variants revealed that the low metastatic cells contained more galactose/N-acetylgalactosamine residues in a high-molecular-mass (15-20 kDa) mixed N- and O-glycan fraction than the highly metastatic ones. It was also found that the highly met...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 1990-01, Vol.116 (3), p.264-270
Main Authors: TIMAR, J, LADANYI, A, LAPIS, K, MOCZAR, E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cytotoxicity, Immunologic - drug effects
Galactose - analysis
Lung Neoplasms - analysis
Lung Neoplasms - immunology
Macrophages - immunology
Medical sciences
Mice
Mice, Inbred C57BL
Neoplasm Metastasis
Oligosaccharides - pharmacology
Phenotype
Pneumology
Polysaccharides - analysis
Tumors of the respiratory system and mediastinum
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Summary:Biochemical and cytochemical analysis of Lewis lung tumor variants revealed that the low metastatic cells contained more galactose/N-acetylgalactosamine residues in a high-molecular-mass (15-20 kDa) mixed N- and O-glycan fraction than the highly metastatic ones. It was also found that the highly metastatic variant was less sensitive to macrophage cytotoxicity in vitro. The cytotoxicity against the low metastatic target cells was inhibited by asialofetuin (10-20 microM), and, to a small degree--and at much higher concentration--by lactose, while galactose and other monosaccharides were ineffective. We suppose that complex galactosylated tumor cell membrane glycans could play a role in the antitumoral cytotoxicity of macrophages.
ISSN:0171-5216
1432-1335
DOI:10.1007/BF01612901