Sodium dodecyl sulfate–capillary gel electrophoretic analysis of molecular mass microheterogeneity of β-trace protein in cerebrospinal fluid from patients with central nervous system diseases

• Published in: Journal of Chromatography A 1998-04, Vol.802 (1), p.143-148
• Main Authors: Hiraoka, Atsushi, Arato, Teruyo, Tominaga, Itaru, Eguchi, Naomi, Oda, Hiroshi, Urade, Yoshihiro
• Format: Article
• Language: English
• Subjects: Adult; Aged; Beta-Globulins - cerebrospinal fluid; Biological and medical sciences; Central Nervous System Diseases - cerebrospinal fluid; Central Nervous System Diseases - diagnosis; Cerebrospinal Fluid Proteins - analysis; Cohort Studies; Electrophoresis, Capillary - methods; Female; Humans; Intramolecular Oxidoreductases; Investigative techniques, diagnostic techniques (general aspects); Lipocalins; Male; Medical sciences; Middle Aged; Myelin Basic Protein - cerebrospinal fluid; Nervous system; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Proteins; Sodium Dodecyl Sulfate - chemistry; Spinal Puncture; β-Trace proteins
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(97)00909-6

Molecular mass ( M r) microheterogeneity of β-trace protein (βTP) in cerebrospinal fluid (CSF) from patients with various neurological disorders was analyzed by sodium dodecyl sulfate capillary gel electrophoresis. Under the conditions employed, βTP with a M r distribution of 23 000–30 000 was roughly separated into two subfractions containing the major peaks with M r of 26 000 and 28 500, respectively. The peak area ratios of the two subfractions on the electropherograms varied among the samples examined, and elevation in the total βTP level in the CSF from patients with organic diseases in the central nervous system (CNS) was often accompanied by changes in the ratios of the subfractions. The quantitative changes in the subfraction level in CSF βTP are considered to reflect the pathological alterations in the CNS.