Genomic Diversity among Streptococcus agalactiae Isolates Detected by a Degenerate Oligonucleotide-Primed Amplification Assay

A random-amplified polymorphic DNA assay using partially degenerate oligonucleotides as primers was used for the characterization of 78 epidemiologically related and unrelated clinical isolates of Streptococcus agalactiae belonging to different serotypes. Thirty distinct amplification profiles were...

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Bibliographic Details
Published in:The Journal of infectious diseases 1998-05, Vol.177 (5), p.1308-1313
Main Authors: Limansky, Adriana S., Sutich, Emma G., Guardati, María C., Toresani, Inés E., Viale, Alejandro M.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
DNA
DNA Primers
DNA, Bacterial - chemistry
DNA, Bacterial - isolation & purification
Fundamental and applied biological sciences. Psychology
Genetic polymorphism
Genetic Variation
Genomics
Genotype
Humans
Infections
Major Articles
Microbiology
Nucleotides
Oligonucleotides
Polymerase chain reaction
Polymerase Chain Reaction - methods
Random Amplified Polymorphic DNA Technique
Reinfection
Serotyping
Streptococcal Infections - virology
Streptococcus agalactiae
Streptococcus agalactiae - classification
Streptococcus agalactiae - genetics
Streptococcus agalactiae - isolation & purification
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Summary:A random-amplified polymorphic DNA assay using partially degenerate oligonucleotides as primers was used for the characterization of 78 epidemiologically related and unrelated clinical isolates of Streptococcus agalactiae belonging to different serotypes. Thirty distinct amplification profiles were obtained among 52 unrelated S. agalactiae isolates assigned to nine groups by serotyping (including 3 nontypeable strains), uncovering the extent of genomic heterogeneity existent within serotypes. This method was particularly useful in providing evidence for or against vertical transmission of a given clone of this microorganism, as well as for relapsing or reinfection in related cases, and suggested clonal relatedness between unrelated S. agalactiae isolates associated with some invasive infections. Thus, this simple methodology represents a suitable tool for the epidemiologic study of S. agalactiae infections.
ISSN:0022-1899
1537-6613
DOI:10.1086/515275