Naive Human T Cells Develop into Th1 or Th0 Effectors and Exhibit Cytotoxicity Early after Stimulation with Leishmania-Infected Macrophages

Studies of human disease suggest that naturally acquired immunity is the predominant outcome of Leishmania infection. Normally protective immune mechanisms activated during asymptomatic or self-healing infections may be minimal in patients who develop disease. To explore early immune responses, an i...

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Bibliographic Details
Published in:The Journal of infectious diseases 1998-05, Vol.177 (5), p.1345-1351
Main Authors: Russo, Donna M., Chakrabarti, Pampa, Burns, James M.
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
Biological and medical sciences
Cell Differentiation
Cell Line
Cell lines
Cells, Cultured
Cultured cells
Cytokines
Cytokines - biosynthesis
Cytotoxicity
Cytotoxicity, Immunologic
Human protozoal diseases
Humans
Infections
Infectious diseases
Interleukin-12 - biosynthesis
Interleukin-12 - pharmacology
Interleukin-2 - pharmacology
Leishmania - immunology
Leishmaniasis
Leshmaniasis
Macrophages
Macrophages - cytology
Macrophages - immunology
Macrophages - parasitology
Major Articles
Medical sciences
Parasitic diseases
Phenotypes
Protozoal diseases
T lymphocytes
T-Lymphocytes - cytology
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
T-Lymphocytes, Helper-Inducer - cytology
T-Lymphocytes, Helper-Inducer - immunology
Th1 Cells - cytology
Th1 Cells - immunology
Transponders
Tropical medicine
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Summary:Studies of human disease suggest that naturally acquired immunity is the predominant outcome of Leishmania infection. Normally protective immune mechanisms activated during asymptomatic or self-healing infections may be minimal in patients who develop disease. To explore early immune responses, an in vitro model of human Leishmania infection was developed in which naive T cells were sensitized with Leishmania-infected macrophages. An analysis of Leishmania-specific cytokine production by these T cell lines revealed that most individuals developed Th1 or Th0 responses early after infection. Infected macrophages from Th1 responders produced interleukin-12. Th0 responders who produced little or no endogenous interleukin-12 could be converted to the Th1 phenotype by addition of interleukin-12 during priming. Finally, infection-sensitized T cells specifically lysed Leishmania-infected macrophages. Thus, this in vitro model system can be used to delineate protective human immune responses against Leishmania induced early after infection.
ISSN:0022-1899
1537-6613
DOI:10.1086/515284