6-C-kine (SLC), a lymphocyte adhesion-triggering chemokine expressed by high endothelium, is an agonist for the MIP-3beta receptor CCR7

The beta chemokine known as 6-C-kine, secondary lymphoid-tissue chemokine (SLC), TCA4, or Exodus-2 (herein referred to as 6CK/SLC) can trigger rapid integrin-dependent arrest of lymphocytes rolling under physiological shear and is highly expressed by high endothelial venules, specialized vessels inv...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 1998-05, Vol.141 (4), p.1053-1059
Main Authors: Campbell, J J, Bowman, E P, Murphy, K, Youngman, K R, Siani, M A, Thompson, D A, Wu, L, Zlotnik, A, Butcher, E C
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion
Cells, Cultured
Cellular biology
Chemokine CCL19
Chemokine CCL21
Chemokines, CC - biosynthesis
Chemokines, CC - pharmacology
Chemokines, CC - physiology
Chemotaxis, Leukocyte - drug effects
Chemotaxis, Leukocyte - physiology
Endothelium, Vascular - physiology
Humans
Immunology
Lymphocyte Subsets - physiology
Lymphocytes - physiology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Receptors, CCR7
Receptors, Chemokine - agonists
Receptors, Chemokine - biosynthesis
Receptors, Chemokine - physiology
Recombinant Proteins - biosynthesis
Species Specificity
Transfection
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The beta chemokine known as 6-C-kine, secondary lymphoid-tissue chemokine (SLC), TCA4, or Exodus-2 (herein referred to as 6CK/SLC) can trigger rapid integrin-dependent arrest of lymphocytes rolling under physiological shear and is highly expressed by high endothelial venules, specialized vessels involved in lymphocyte homing from the blood into lymph nodes and Peyer's patches. We show that 6CK/SLC is an agonist for the lymphocyte chemoattractant receptor, CCR7 (EBI-1, BLR-2), previously described as a receptor for the related beta chemokine MIP-3beta (ELC or Exodus-3). Moreover, 6CK/SLC and MIP-3beta attract the same major populations of circulating lymphocytes, including naive and memory T cells > B cells (but not natural killer cells); desensitization to MIP-3beta inhibits lymphocyte chemotaxis to 6CK/SLC but not to the alpha chemokine SDF-1 (stromal cell-derived factor); and 6CK/SLC competes for MIP-3beta binding to resting mouse lymphocytes. The findings suggest that the majority of circulating lymphocytes respond to 6CK/SLC and MIP-3beta in large part through their common receptor CCR7 and that these molecules may be important mediators of physiological lymphocyte recirculation in vivo.
ISSN:0021-9525
1540-8140