Mapping of the human and murine X11-like genes (APBA2 and apba2), the murine Fe65 gene (Apbb1), and the human Fe65-like gene (APBB2): genes encoding phosphotyrosine-binding domain proteins that interact with the Alzheimer's disease amyloid precursor protein

• Published in: Mammalian genome 1998-06, Vol.9 (6), p.473-475
• Main Authors: Blanco, G, Irving, N G, Brown, S D, Miller, C C, McLoughlin, D M
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing; Alzheimer Disease - metabolism; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - metabolism; Animals; Binding Sites - genetics; Chromosome Mapping; Humans; Mice; Mice, Inbred C57BL; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Nuclear Proteins - metabolism; Phosphotyrosine - metabolism; Protein Binding
• ISSN: 0938-8990; 1432-1777
• DOI: 10.1007/s003359900800

In order to map the human APBA2 and APBB2 genes, we selected PCR primers from the previously identified cDNA clones and overlapping sequences deposited in the databases (accession numbers R89683, R13010, R18654, and T16098 for APBA2 and accession number HSU62325 for APBB2). For APBA2 the following primer pair: forward, 5'-TTACAAGTCGTGTCCTGGGAG-3', and reverse, 5'-GACGTCTGGGGTCCTGTG-3', generated a small PCR product of 103 bp. For APBB2 the following primer pair: forward, 5'-CACAGAGAAGAGTCTGGCCC-3' and reverse, 5'-AGGTTGCTTGTGACAGGTCC-3', generated a PCR product of 114 bp. These PCR products were sequenced to confirm they originated from the correct genes. Both human APBA2 and APBB2 genes were mapped using the Genebridge 4 radiation hybrid panel (HGMP Resource Centre, Cambridge, UK) consisting of 94 hamster-derived cell lines. PCR amplification of human DNA with PCR primers designed for these genes resulted in products of the expected size, while no amplification products were obtained from the hamster DNA control sample. Scores for individual cell lines were submitted at the WICGR mapping service at http://www.genome.wi.mit.edu. APBA2 was assigned to human Chr 15 between the markers WI-5590 (10.31 cR) and D15S144 (21.7 cR). APBB2 was assigned to human Chr 4 between the markers D4S405 (4.6 cR) and D4S496 (10.1 cR). To map the Apba2 and Apbb1 loci in the mouse, we used the EUCIB resource which comprises 982 interspecific backcross progeny for high-resolution genetic mapping across the mouse genome.