Downregulation of Insulin-like Growth Factor-1 Receptor (IGF-1R) Expression in Human T Lymphocyte Activation

Although it is known that IGF-1 increases T lymphocyte proliferation, the regulation of its receptor expression during the process has not been defined. Consequently the regulation of IGF-1R expression by IGF-1 and the activation events in human blood T lymphocytes and the Jurkat T cell line were no...

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Bibliographic Details
Published in:Cellular immunology 1998-02, Vol.183 (2), p.157-161
Main Authors: Schillaci, Roxana, Brocardo, Mariana G., Galeano, Adriana, Roldán, Alicia
Format: Article
Language:English
Subjects:
Down-Regulation
Flow Cytometry
Humans
Insulin-Like Growth Factor I - pharmacology
Jurkat Cells
Lymphocyte Activation
Receptor, IGF Type 1 - biosynthesis
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
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Summary:Although it is known that IGF-1 increases T lymphocyte proliferation, the regulation of its receptor expression during the process has not been defined. Consequently the regulation of IGF-1R expression by IGF-1 and the activation events in human blood T lymphocytes and the Jurkat T cell line were now investigated. IGF-1R expression in nonstimulated Jurkat cells was confirmed and downregulation by IGF-1 was demonstrated. In addition, both cell types showed a time-dependent reduction in the number of IGF-1R-positive cells following activation, which was increased by IGF-1. In Jurkat cells the negative regulation of IGF-1R levels was correlated with the appearance and continuous increase in IL-2R. In T lymphocytes the decrease in IGF-1R expression was faster, reaching its plateau after 3 h of activation. Our findings suggest that loss of the IGF-1R is one of the initial events of the activation process not regulated by IL-2.
ISSN:0008-8749
1090-2163
DOI:10.1006/cimm.1997.1237