Changes of Gene Expression by Lysophosphatidylcholine in Vascular Endothelial Cells: 12 Up-Regulated Distinct Genes Including 5 Cell Growth-Related, 3 Thrombosis-Related, and 4 Others

Lysophosphatidylcholine (lysoPC), a component of oxidatively modified lipoproteins, is present in atherosclerotic lesions, and its proatherogenic properties have been demonstrated. To gain an insight into lysoPC-mediated endothelial gene expression, we applied nonradioactive differential display ana...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1998-06, Vol.123 (6), p.1119-1126
Main Authors: Sato, Naoaki, Kokame, Koichi, Shimokado, Kentaro, Kato, Hisao, Miyata, Toshiyuki
Format: Article
Language:English
Subjects:
A Kinase Anchor Proteins
Amino Acid Sequence
Animals
atherosclerosis
Base Sequence
Cell Cycle Proteins
Cell Division - genetics
Cells, Cultured
DNA, Complementary - analysis
DNA, Complementary - genetics
Endothelium, Vascular - cytology
Endothelium, Vascular - physiology
Gene Expression Regulation - drug effects
gravin
growth regulation
Humans
Lysophosphatidylcholines - pharmacology
MAP kinase
Molecular Sequence Data
oxidized LDL
Protein Biosynthesis
Proteins - genetics
Rats
Thrombosis - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lysophosphatidylcholine (lysoPC), a component of oxidatively modified lipoproteins, is present in atherosclerotic lesions, and its proatherogenic properties have been demonstrated. To gain an insight into lysoPC-mediated endothelial gene expression, we applied nonradioactive differential display analysis of mRNA from lysoPC-treated and untreated human umbilical vein endothelial cells. We identified 12 up-regulated distinct genes including 5 cell growth-related genes (two phosphatases CL100 and B23/hVH-3, gravin, activating transcription factor-4, and heparin-binding epidermal growth factor-like growth factor), 3 thrombosis-related genes (plasminogen activator inhibitor-1, tissue plasminogen activator, and thrombomodulin), and 4 others (stanniocalcin, NAD-dependent methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, BENE, and reducing agents and tunicamycin-responsive protein). We isolated a full-length cDNA of human gravin. The cDNA sequence of gravin was homologous with rat mitogenic regulatory gene or rat protein kinase C binding protein and substrate, suggesting that gravin would regulate cell growth. Thus, lysoPC apparently accelerates atherosclerosis by regulating the expression of a wide variety of genes. Our data suggest the involvement in atherogenesis of the genes hitherto regarded as atherosclerosis-unrelated.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a022051