JC Virus Remains Latent in Peripheral Blood B Lymphocytes but Replicates Actively in Urine from AIDS Patients

JC virus (JCV) is thought to reach the central nervous system by a vascular route. To determine whether JCV is conveyed in peripheral blood as latent or reactivated virus, blood leukocytes, plasma, and urine from 50 AIDS patients and plasma and B lymphocytes from 60 AIDS patients were investigated....

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Bibliographic Details
Published in:The Journal of infectious diseases 1998-06, Vol.177 (6), p.1502-1505
Main Authors: Lafon, Marie-Edith, Dutronc, Hervé, Véronique, Dubois, Pellegrin, Isabelle, Barbeau, Pascal, Ragnaud, Jean-Marie, Pellegrin, Jean-Luc, Fleury, Hervé J. A.
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - complications
Acquired Immunodeficiency Syndrome - urine
Acquired Immunodeficiency Syndrome - virology
Adult
Aged
AIDS
AIDS/HIV
B lymphocytes
B-Lymphocytes - virology
Biological and medical sciences
Blood
Blood plasma
CD4 Lymphocyte Count
DNA
DNA, Viral
Female
Human viral diseases
Humans
Infectious diseases
JC virus
JC Virus - genetics
JC Virus - isolation & purification
JC Virus - physiology
Leukocytes
Major Articles
Male
Medical sciences
Messenger RNA
Middle Aged
Papillomavirus Infections - urine
Papillomavirus Infections - virology
Polymerase Chain Reaction
RNA, Messenger
RNA, Viral
Tumor Virus Infections - urine
Tumor Virus Infections - virology
Urine
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Load
Virus Latency
Virus Replication
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Summary:JC virus (JCV) is thought to reach the central nervous system by a vascular route. To determine whether JCV is conveyed in peripheral blood as latent or reactivated virus, blood leukocytes, plasma, and urine from 50 AIDS patients and plasma and B lymphocytes from 60 AIDS patients were investigated. Peripheral blood from 88 human immunodeficiency virus-negative blood donors was studied. Nested polymerase chain reaction assays allowed the identification of JCV T DNA and VP1 mRNAs. The latter indicate viral replication. Blood harbored JCV DNA in 31.8% of AIDS patients (only 2.3% of blood donors; P>.001) and urine in 56%. VP1 mRNAs were detected in blood of 1 AIDS patient. Notably, 38% of DNA-positive urine samples and 10 cerebrospinal fluid samples (CSF) from AIDS patients with progressive multifocal leukoencephalopathy contained JCV mRNAs. Thus, JCV was significantly more frequent in blood from AIDS patients than from controls, but, in most instances, it was latent, whereas active replication was detected in urine and CSF.
ISSN:0022-1899
1537-6613
DOI:10.1086/515305