DNA extraction from archival Giemsa‐stained bone‐marrow slides: comparison of six rapid methods

The ability of six rapid DNA extraction procedures to provide DNA for the polymerase chain reaction from archival Giemsa‐stained bone marrow slides was tested on 120 samples. Boiling in distilled water, freeze–thaw method, boiling in 10% Chelex‐100 resin solution, proteinase K/Tween 20/NP‐40 method...

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Bibliographic Details
Published in:British journal of haematology 1998-05, Vol.101 (2), p.349-351
Main Authors: Vince, Adriana, Poljak, Mario, Seme, Katja
Format: Article
Language:English
Subjects:
amplification
Azure Stains
Biological and medical sciences
Bone Marrow Cells - chemistry
bone marrow slides
DNA
DNA - isolation & purification
Giemsa
Hematology
Histocytological Preparation Techniques
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
PCR
Polymerase Chain Reaction - methods
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Summary:The ability of six rapid DNA extraction procedures to provide DNA for the polymerase chain reaction from archival Giemsa‐stained bone marrow slides was tested on 120 samples. Boiling in distilled water, freeze–thaw method, boiling in 10% Chelex‐100 resin solution, proteinase K/Tween 20/NP‐40 method coupled with simplified phenol/chloroform/isoamyl alcohol protocol or salting‐out procedure using saturated NaCl and modification of commercial QIAamp procedure (Qiagen, Chatsworth, Calif.) gave DNA extraction efficiencies of 50%, 70%, 85%, 95%, 100% and 100%, respectively. Our results demonstrate that rough DNA extraction methods have decreased efficiencies compared to complete DNA extraction protocols and that the latter are required to ensure highly reproducible results from archival Giemsa‐stained bone marrow slides.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1998.00702.x