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Separation of metalloprotein complexes in serum by size-exclusion chromatography: Optimisation of the separation parameters retention behaviour and recovery employing radiotracers
A suitable procedure was developed for speciation analysis of metalloprotein complexes in serum using directly coupled size-exclusion chromatography and an element-specific detector. Two column matrices used for size-exclusion chromatography (TSK G 3000 SW and Asahipak GS 520) were investigated with...
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| Published in: | Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1998-04, Vol.707 (1), p.17-24 |
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| Main Authors: | , |
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| Language: | English |
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| container_end_page | 24 |
| container_issue | 1 |
| container_start_page | 17 |
| container_title | Journal of chromatography. B, Biomedical sciences and applications |
| container_volume | 707 |
| creator | Raab, Andrea Brätter, Peter |
| description | A suitable procedure was developed for speciation analysis of metalloprotein complexes in serum using directly coupled size-exclusion chromatography and an element-specific detector. Two column matrices used for size-exclusion chromatography (TSK G 3000 SW and Asahipak GS 520) were investigated with respect to the recovery and retention behaviour for metalloprotein complexes. Optimisation of the separation parameters (buffer type, concentration, pH) was achieved by means of metalloprotein complexes marked with radiotracers. For speciation of serum the matrix in the Asahipak GS column is more efficient. Given optimal eluent characteristics (100 m
M Tris, pH 7.4) the recovery of the elements investigated (sodium, calcium, iron and zinc) was 100%. Further, the retention behaviour (retention time, ratios of the peak areas) remained unchanged for several successive separations. |
| doi_str_mv | 10.1016/S0378-4347(97)00613-0 |
| format | article |
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M Tris, pH 7.4) the recovery of the elements investigated (sodium, calcium, iron and zinc) was 100%. Further, the retention behaviour (retention time, ratios of the peak areas) remained unchanged for several successive separations.</description><identifier>ISSN: 0378-4347</identifier><identifier>ISSN: 1387-2273</identifier><identifier>DOI: 10.1016/S0378-4347(97)00613-0</identifier><identifier>PMID: 9613929</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Buffers ; Calcium - blood ; Calcium - isolation & purification ; Chromatography, Gel ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrogen-Ion Concentration ; Indicators and Reagents ; Metalloprotein ; Metalloproteins ; Metalloproteins - blood ; Metalloproteins - isolation & purification ; Other metalloproteins ; Proteins ; Silicon Dioxide ; Sodium - blood ; Sodium - isolation & purification ; Spectrophotometry, Ultraviolet ; Zinc - blood ; Zinc - isolation & purification</subject><ispartof>Journal of chromatography. B, Biomedical sciences and applications, 1998-04, Vol.707 (1), p.17-24</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2224582$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9613929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raab, Andrea</creatorcontrib><creatorcontrib>Brätter, Peter</creatorcontrib><title>Separation of metalloprotein complexes in serum by size-exclusion chromatography: Optimisation of the separation parameters retention behaviour and recovery employing radiotracers</title><title>Journal of chromatography. B, Biomedical sciences and applications</title><addtitle>J Chromatogr B Biomed Sci Appl</addtitle><description>A suitable procedure was developed for speciation analysis of metalloprotein complexes in serum using directly coupled size-exclusion chromatography and an element-specific detector. Two column matrices used for size-exclusion chromatography (TSK G 3000 SW and Asahipak GS 520) were investigated with respect to the recovery and retention behaviour for metalloprotein complexes. Optimisation of the separation parameters (buffer type, concentration, pH) was achieved by means of metalloprotein complexes marked with radiotracers. For speciation of serum the matrix in the Asahipak GS column is more efficient. Given optimal eluent characteristics (100 m
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Psychology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Indicators and Reagents</topic><topic>Metalloprotein</topic><topic>Metalloproteins</topic><topic>Metalloproteins - blood</topic><topic>Metalloproteins - isolation & purification</topic><topic>Other metalloproteins</topic><topic>Proteins</topic><topic>Silicon Dioxide</topic><topic>Sodium - blood</topic><topic>Sodium - isolation & purification</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Zinc - blood</topic><topic>Zinc - isolation & purification</topic><toplevel>online_resources</toplevel><creatorcontrib>Raab, Andrea</creatorcontrib><creatorcontrib>Brätter, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Biomedical sciences and applications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raab, Andrea</au><au>Brätter, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of metalloprotein complexes in serum by size-exclusion chromatography: Optimisation of the separation parameters retention behaviour and recovery employing radiotracers</atitle><jtitle>Journal of chromatography. B, Biomedical sciences and applications</jtitle><addtitle>J Chromatogr B Biomed Sci Appl</addtitle><date>1998-04-10</date><risdate>1998</risdate><volume>707</volume><issue>1</issue><spage>17</spage><epage>24</epage><pages>17-24</pages><issn>0378-4347</issn><issn>1387-2273</issn><abstract>A suitable procedure was developed for speciation analysis of metalloprotein complexes in serum using directly coupled size-exclusion chromatography and an element-specific detector. Two column matrices used for size-exclusion chromatography (TSK G 3000 SW and Asahipak GS 520) were investigated with respect to the recovery and retention behaviour for metalloprotein complexes. Optimisation of the separation parameters (buffer type, concentration, pH) was achieved by means of metalloprotein complexes marked with radiotracers. For speciation of serum the matrix in the Asahipak GS column is more efficient. Given optimal eluent characteristics (100 m
M Tris, pH 7.4) the recovery of the elements investigated (sodium, calcium, iron and zinc) was 100%. Further, the retention behaviour (retention time, ratios of the peak areas) remained unchanged for several successive separations.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9613929</pmid><doi>10.1016/S0378-4347(97)00613-0</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0378-4347 |
| ispartof | Journal of chromatography. B, Biomedical sciences and applications, 1998-04, Vol.707 (1), p.17-24 |
| issn | 0378-4347 1387-2273 |
| language | eng |
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| source | Elsevier |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Buffers Calcium - blood Calcium - isolation & purification Chromatography, Gel Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration Indicators and Reagents Metalloprotein Metalloproteins Metalloproteins - blood Metalloproteins - isolation & purification Other metalloproteins Proteins Silicon Dioxide Sodium - blood Sodium - isolation & purification Spectrophotometry, Ultraviolet Zinc - blood Zinc - isolation & purification |
| title | Separation of metalloprotein complexes in serum by size-exclusion chromatography: Optimisation of the separation parameters retention behaviour and recovery employing radiotracers |
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