Use of Nested PCR to Identify Charred Human Remains and Minute Amounts of Blood

Reliable single cell PCR requires nested or heminested PCR and careful optimization of conditions. This report describes the successful use of nested PCR for gender identification and reverse paternity testing in a forensic case where the only available materials consisted of charred human remains a...

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Bibliographic Details
Published in:Journal of forensic sciences 1998-05, Vol.43 (3), p.696-700
Main Authors: Strom, CM, Rechitsky, S
Format: Article
Language:English
Subjects:
Amelogenin
Apolipoproteins B - genetics
Autopsy
Blood
Blood Stains
Burns
Cadaver
Dental Enamel Proteins - genetics
Deoxyribonucleic acid
DNA
DNA - genetics
DNA Fingerprinting
DNA Primers - chemistry
Enzymes
Female
Forensic sciences
Homicide
Human remains
Humans
Male
Paternity
Polymerase Chain Reaction - methods
Sex Determination Analysis - methods
von Willebrand Factor - genetics
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Summary:Reliable single cell PCR requires nested or heminested PCR and careful optimization of conditions. This report describes the successful use of nested PCR for gender identification and reverse paternity testing in a forensic case where the only available materials consisted of charred human remains and a minute quantity of blood that were unsuitable for standard PCR. Use of nested PCR allowed the blood and burned tissue to be identified as human female. Analysis of two PCR length polymorphisms (AMPFLP) was successful on the blood sample and reverse paternity testing yielded a 98% probability that the blood spot was from the victim. The defendant was convicted of murder following a bench trial and the verdict was upheld by the Appellate court.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS16204J