Abnormal content of n−6 and n−3 long-chain unsaturated fatty acids in the phosphoglycerides and cholesterol esters of parahippocampal cortex from Alzheimer's disease patients and its relationship to acetyl CoA content

• Published in: The international journal of biochemistry & cell biology 1998-02, Vol.30 (2), p.197-207
• Main Authors: Corrigan, F.M., Horrobin, D.F., Skinner, E.R., Besson, J.A.O., Cooper, M.B.
• Format: Article
• Language: English
• Subjects: Acetyl CoA; Acetyl Coenzyme A - analysis; Adrenic acid; Aged; Aged, 80 and over; Alzheimer Disease - enzymology; Alzheimer Disease - metabolism; Alzheimer's disease; Arachidonic acid; Cholesterol Esters - chemistry; Cholesterol Esters - metabolism; Chromatography, Gas; Fatty Acids - analysis; Fatty Acids, Omega-3 - analysis; Fatty Acids, Omega-6; Fatty Acids, Unsaturated - analysis; Female; Glycerophosphates - chemistry; Glycerophosphates - metabolism; Hippocampus - metabolism; Humans; Male; Middle Aged; Parahippocampus; Phosphatidylcholines - chemistry; Phosphatidylethanolamines - chemistry; Phosphatidylinositols - chemistry; Phosphatidylserines - chemistry
• ISSN: 1357-2725; 1878-5875
• DOI: 10.1016/S1357-2725(97)00125-8

The long-chain fatty acid composition of cholesterol esters, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI) from parahippocampal cortex of Alzheimer's disease (AD) patients and control subjects was examined. In general the PC fraction contained less polyunsaturated long-chain fatty acids than did PE, PS or PI. Of the n−6 polyunsaturated long-chain fatty acids, PI contained the greatest incorporation of these acids followed by PE. There were significant differences between controls and AD patients in total n−6 EFAs. Arachidonic acid (C20:4 n−6) was the predominant fatty acid of this family found to be present. In AD, PE and PS showed a deficit of adrenic acid (C22:4 n−6) content and PE also contained less arachidonic acid. In AD subjects, the cholesterol esters contained significantly less n−3 polyunsaturated fatty acids with, specifically, a reduction in alpha-linolenic acid. Acetyl CoA content of hippocampal cortex was greater in AD patients than in control subjects indicating either an increased extent of oxidative metabolism or a failure to utilise acetyl CoA for anabolic processes. Abnormal magnitude of oxidative processes could give rise to the biosynthesis of PE and PS species containing less n−6 polyunsaturated fatty acids than occurs in control subjects.