Cloning and Characterization of the Mouse and Rat Type II Arginase Genes

Two forms of arginase, both catalyzing the hydrolysis of arginine to ornithine and urea, are found in animals ranging from amphibians to mammals. In humans, inherited deficiency of hepatic or type I arginase results in hyperargininemia, a syndrome characterized by periodic episodes of hyperammonemia...

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Bibliographic Details
Published in:Molecular genetics and metabolism 1998-03, Vol.63 (3), p.168-175
Main Authors: Iyer, Ramaswamy K., Bando, Joanne M., Jenkinson, Christopher P., Vockley, Joseph G., Kim, Phillip S., Kern, Rita M., Cederbaum, Stephen D., Grody, Wayne W.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
arginase
Arginase - chemistry
Arginase - genetics
Arginase - metabolism
Cloning, Molecular
DNA, Complementary
Escherichia coli - metabolism
Female
Humans
in vitroexpression
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Phylogeny
Precipitin Tests
Rats
Rats, Sprague-Dawley
Sequence Homology, Amino Acid
Urea - metabolism
urea cycle
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Summary:Two forms of arginase, both catalyzing the hydrolysis of arginine to ornithine and urea, are found in animals ranging from amphibians to mammals. In humans, inherited deficiency of hepatic or type I arginase results in hyperargininemia, a syndrome characterized by periodic episodes of hyperammonemia, spasticity, and neurological deterioration. In these patients, a second extrahepatic or type II arginase activity is significantly increased, an induction that may partially compensate for the lack of AI activity and apparently mitigates some of the clinical effects of the condition. Cloning and characterization of the human AII cDNA was recently accomplished. The cloning, sequencing, and partial characterization of the mouse and rat AII cDNAs are reported herein. The DNA sequences predicted polypeptides of 354 amino acids, including a N-terminal mitochondrial import signal. Sequence homology to the human type II arginase, arginase activity data, and immunoprecipitation with an anti-AII antibody confirm the identity of these cloned genes as rodent extrahepatic type II arginases.
ISSN:1096-7192
1096-7206
DOI:10.1006/mgme.1997.2669