Oriented immobilization of bacterial photosynthetic membrane

We have examined a method for oriented immobilization of photosynthetic membrane fragments on a solid surface by specific avidin-biotin interaction. Photosynthetic membrane fragments from the purple non-sulphur photosynthetic bacterium Rhodopseudomonas viridis, of which the H-subunit of the photosyn...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1990-02, Vol.32 (5), p.544-549
Main Authors: HARA, M, MAJIMA, T, MIYAKE, J, AJIKI, S, SUGINO, H, TOYOTAMA, H, KAWAMURA, S
Format: Article
Language:English
Subjects:
Adsorption
Antibodies, Bacterial - immunology
Bacterial Proteins - isolation & purification
Bacterial Proteins - metabolism
Bacteriological Techniques
Biological and medical sciences
Biotechnology
Biotin
Cell Membrane - physiology
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
immobilization
Immobilization of organelles and whole cells
Immobilization techniques
Light
Membrane Potentials
membranes
Methods. Procedures. Technologies
photosynthesis
Photosynthesis - physiology
Photosynthetic Reaction Center Complex Proteins
Rhodopseudomonas - physiology
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Summary:We have examined a method for oriented immobilization of photosynthetic membrane fragments on a solid surface by specific avidin-biotin interaction. Photosynthetic membrane fragments from the purple non-sulphur photosynthetic bacterium Rhodopseudomonas viridis, of which the H-subunit of the photosynthetic reaction centre was biotinylated, was immobilized on an avidin-adsorbed plate. Orientation of the immobilized membrane on the plastic plate was checked by an antisera binding assay that could react to the respective sides of the membrane: the H-subunit side was selectively adsorbed on the plate. Light-induced potential and current responses could be measured when the membrane immobilized on the SnO2-coated glass plate was dried and sandwiched with a counter electrode of Hg. The electrical response in the immobilized membrane was much improved in comparison with the control (membranes were simply adsorbed on the plate), supporting the idea that the membranes have an orientation on solid surfaces.
ISSN:0175-7598
1432-0614
DOI:10.1007/bf00173725