Molecular Characterization of Low-Molecular-Weight Component Protein, Flp, in Actinobacillus actinomycetemcomitans Fimbriae

• Published in: MICROBIOLOGY and IMMUNOLOGY 1998, Vol.42(4), pp.253-258
• Main Authors: Inoue, Tetsuyoshi, Tanimoto, Ichiro, Ohta, Hiroyuki, Kato, Keijiro, Murayama, Yoji, Fukui, Kazuhiro
• Format: Article
• Language: English
• Subjects: Actinobacillus actinomycetemcomitans; Aggregatibacter actinomycetemcomitans - chemistry; Aggregatibacter actinomycetemcomitans - genetics; Aggregatibacter actinomycetemcomitans - ultrastructure; Amino Acid Sequence; Bacterial Proteins - analysis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacteriology; Base Sequence; Biological and medical sciences; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Fimbriae; Fimbriae, Bacterial - chemistry; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Humans; Immunohistochemistry; Microbiology; Microscopy, Electron; Molecular Sequence Data; Morphology, structure, chemical composition; Sequence Analysis, DNA; Sequence Homology, Amino Acid
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1998.tb02280.x

Fimbriae preparation from Actinobacillus actinomycetemcomitans was found to contain an abundant low-molecular-weight protein (termed Flp) with an apparent molecular mass of approximately 6.5kDa, in addition to a small amount of 54-kDa protein. Immunogold electron microscopy localized the Flp protein at the bacterial fimbriae but not at the cell surface. The DNA fragment including the flp gene was cloned from A. actinomycetemcomitans 304-a and its nucleotide sequence was determined. An open reading frame of the flp gene was composed of 225bp encoding a protein of 75 amino acids. Comparison of the translated amino acid sequence with the sequence of native Flp determined by Edman degradation indicated that the N-terminal part of 26 amino acids is leader peptide. The N-terminal sequence of mature Flp exhibited some similarity to type-IV pilin. Furthermore, the processing site of premature Flp is also similar to that of type-IV prepilin, and a gene encoding a protein homologous to type-IV prepilin-like protein leader peptidase was found downstream of the flp gene. These findings indicate that Flp is the major component protein of A. actinomycetemcomitans fimbriae.