Platelet-activating factor induces goblet cell hyperplasia and mucin gene expression in airways

In patients dying from asthma, extensive mucous plugging occurs in the airways, associated with goblet cell hyperplasia. In this study, we examined the hypothesis that platelet-activating factor (PAF) induces goblet cell hyperplasia and mucin gene expression. After instilling PAF into the airways of...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 1998-06, Vol.157 (6), p.1927-1934
Main Authors: LOU, Y.-P, TAKEYAMA, K, GRATTAN, K. M, LAUSIER, J. A, UEKI, I. F, AGUSTI, C, NADEL, J. A
Format: Article
Language:English
Subjects:
Animals
Asthma - physiopathology
Biological and medical sciences
Bronchi - pathology
Chronic obstructive pulmonary disease, asthma
Gene Expression Regulation
Guinea Pigs
Hyperplasia
In Situ Hybridization
Male
Medical sciences
Mucins - genetics
Mucous Membrane - pathology
Platelet Activating Factor - physiology
Pneumology
Rats
Rats, Inbred F344
Trachea - pathology
Tumor Necrosis Factor-alpha - pharmacology
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Summary:In patients dying from asthma, extensive mucous plugging occurs in the airways, associated with goblet cell hyperplasia. In this study, we examined the hypothesis that platelet-activating factor (PAF) induces goblet cell hyperplasia and mucin gene expression. After instilling PAF into the airways of guinea pigs and rats, we stained airway goblet cells with Alcian blue/periodic acid-Schiff and determined the number of goblet cells and percentage of stained area within the epithelium. In guinea pigs, one instillation of PAF (10(-)5 M, 100 microl) increased the goblet cell-stained area time-dependently, beginning at 24 h, maximum at 72 h. PAF also caused tracheal recruitment of eosinophils by 24 h, maximum at 48 h. In rats, which have few goblet cells in airways, PAF (3 instillations, 10(-)5 M, 200 microl) caused striking goblet cell hyperplasia, greatest in peripheral airways. Tumor necrosis factor alpha (TNFalpha) alone had no significant effect on goblet cells, but together with PAF, it caused exaggerated goblet cell hyperplasia. In rat tracheas studied by in situ hybridization, PAF induced mucin MUC5 gene expression in epithelial cells that stained for mucosubstances. In summary, PAF induces goblet cell hyperplasia and TNFalpha potentiates this effect.
ISSN:1073-449X
1535-4970
DOI:10.1164/ajrccm.157.6.9709113