A fruit-specific putative dihydroflavonol 4-reductase gene is differentially expressed in strawberry during the ripening process

• Published in: Plant physiology (Bethesda) 1998-06, Vol.117 (2), p.711-716
• Main Authors: Moyano, E, Portero-Robles, I, Medina-Escobar, N, Valpuesta, V, Munoz-Blanco, J, Caballero, J.L
• Format: Article
• Language: English
• Subjects: Achenes; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADN; Agronomy. Soil science and plant productions; Alcohol Oxidoreductases - biosynthesis; Alcohol Oxidoreductases - chemistry; Alcohol Oxidoreductases - genetics; Amino Acid Sequence; AMINO ACID SEQUENCES; Amino acids; ANA; ANTHOCYANE; ANTHOCYANINS; ANTIRRHINUM; ANTIRRHINUM MAJUS; ANTOCIANINAS; ARABIDOPSIS THALIANA; Auxins; Base Sequence; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; CHEMICAL COMPOSITION; Classical and quantitative genetics. Population genetics. Molecular genetics; CLONE; CLONES; Cloning, Molecular; CODE GENETIQUE; CODIGO GENETICO; COLOR; COLOUR; COMPLEMENTARY DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; COULEUR; DEVELOPMENTAL STAGES; DIANTHUS CARYOPHYLLUS; DNA; DNA, Plant - chemistry; Enzymes; ENZYMIC ACTIVITY; ETAPAS DE DESARROLLO; EXPRESION GENICA; EXPRESSION DES GENES; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; FRAGARIA ANANASSA; Fruit - enzymology; Fruit - genetics; Fruit - physiology; FRUITS; FRUTAS; Fundamental and applied biological sciences. Psychology; GENBANK/AF029685; GENBANK/D85102; GENBANK/P51102; GENBANK/X15536; GENBANK/X15537; GENBANK/X75964; GENBANK/Z67983; GENE; GENE EXPRESSION; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Plant - drug effects; Generalities. Genetics. Plant material; GENES; Genes, Plant; GENETIC CODE; GENETIC REGULATION; GENETICA; GENETICS; Genetics and breeding of economic plants; GENETIQUE; Indoleacetic Acids - pharmacology; LOCALIZATION; MADURAMIENTO; Molecular genetics; MOLECULAR SEQUENCE DATA; MURISSAGE; NAA; NUCLEOTIDE SEQUENCE; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Plants; Rapid Communications; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; RIPENING; RNA; ROSA; ROSA (GENERO); ROSA HYBRIDA; SECUENCIA NUCLEOTIDICA; Sequence Alignment; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; STADE DE DEVELOPPEMENT; TANINOS; TANNIN; TANNINS; TRANSCRIPCION; TRANSCRIPTION; VITIS VINIFERA
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.117.2.711

A cDNA clone encoding a putative dihydroflavonol 4-reductase gene has been isolated from a strawberry (Fragaria x ananassa cv Chandler) DNA subtractive library. Northern analysis showed that the corresponding gene is predominantly expressed in fruit, where it is first detected during elongation (green stages) and then declines and sharply increases when the initial fruit ripening events occur, at the time of initiation of anthocyanin accumulation. The transcript can be induced in unripe green fruit by removing the achenes, and this induction can be partially inhibited by treatment of de-achened fruit with naphthylacetic acid, indicating that the expression of this gene is under hormonal control. We propose that the putative dihydroflavonol 4-reductase gene in strawberry plays a main role in the biosynthesis of anthocyanin during color development at the late stages of fruit ripening; during the first stages the expression of this gene could be related to the accumulation of condensed tannins