dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics

Summary PCR‐based detection of single nucleotide polymorphisms is a powerful tool for the plant geneticist. Cleaved amplified polymorphic sequence analysis is the most widely used approach for the detection of single nucleotide polymorphisms. However, this technique is limited to mutations which cre...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 1998-05, Vol.14 (3), p.387-392
Main Authors: Neff, Michael M., Neff, Joseph D., Chory, Joanne, Pepper, Alan E.
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Base Sequence
Biological and medical sciences
Diverse techniques
DNA Primers
DNA, Plant
Fundamental and applied biological sciences. Psychology
Genetic Markers
Genetic Techniques
Molecular and cellular biology
Point Mutation
Polymerase Chain Reaction
Polymorphism, Genetic
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Summary:Summary PCR‐based detection of single nucleotide polymorphisms is a powerful tool for the plant geneticist. Cleaved amplified polymorphic sequence analysis is the most widely used approach for the detection of single nucleotide polymorphisms. However, this technique is limited to mutations which create or disrupt a restriction enzyme recognition site. This paper presents a modification of this technique where mismatches in a PCR primer are used to create a polymorphism based on the target mutation. This technique is useful for following known mutations in segregating populations and genetic mapping of isolated DNAs used for positional based cloning of new genes. In addition, a computer program has been developed that facilitates the design of these PCR primers.
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313X.1998.00124.x