The ubiquitin-related protein RUB1 and auxin response in Arabidopsis

The AXR1 (auxin-resistant) protein, which has features of the ubiquitin-activating enzyme E1, is required for normal response to the plant hormone auxin in Arabidopsis thaliana. ECR1 functions together with AXR1 to activate members of the RUB/NEDD8 family of ubiquitin-related proteins. Extracts from...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1998-06, Vol.280 (5370), p.1760-1763
Main Authors: Del Pozo, J.C. (Indiana University, Bloomington, IN.), Timpte, C, Tan, S, Callis, J, Estelle, M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antiserum
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins
ARABIDOPSIS THALIANA
Auxin
AUXINAS
AUXINE
AUXINS
Biological and medical sciences
Cell nucleus
Cell Nucleus - metabolism
Classical genetics, quantitative genetics, hybrids
Dithiothreitol - pharmacology
Enzymes
ETAPAS DE DESARROLLO DE LA PLANTA
EXTRACTOS VEGETALES
EXTRAIT D'ORIGINE VEGETALE
Flowers & plants
Fundamental and applied biological sciences. Psychology
Gels
GENE
Gene Expression
GENES
Genetic mutation
Genetics of eukaryotes. Biological and molecular evolution
Growth Substances
Indoleacetic Acids - physiology
Ligases - metabolism
Molecular Sequence Data
Mutation
Physiological aspects
PLANT DEVELOPMENTAL STAGES
PLANT EXTRACTS
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
PLANTULAS
PLANTULE
PROTEINAS
PROTEINE
PROTEINS
Pteridophyta, spermatophyta
Recombinant Fusion Proteins - metabolism
SEEDLINGS
Space life sciences
STADE DE DEVELOPPEMENT VEGETAL
Ubiquitin
Ubiquitin-Activating Enzymes
Ubiquitin-Protein Ligases
Ubiquitins
Ubiquitins - metabolism
Vegetals
Yeasts
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Description
Summary:The AXR1 (auxin-resistant) protein, which has features of the ubiquitin-activating enzyme E1, is required for normal response to the plant hormone auxin in Arabidopsis thaliana. ECR1 functions together with AXR1 to activate members of the RUB/NEDD8 family of ubiquitin-related proteins. Extracts from mutant seedlings lacking AXR1 did not promote formation of the RUB-ECR1 thiolester, indicating that AXR1 is the major activity in this tissue. AXR1 was localized primarily to the nucleus of dividing and elongating cells, suggesting that the targets of RUB modification are nuclear. These results indicate that auxin response depends on RUB modification of one or more nuclear proteins
ISSN:0036-8075
1095-9203
DOI:10.1126/science.280.5370.1760