Construction of a human BcgI DNA fragment library

A library made up of 36 bp DNA fragments generated by digestion of human DNA with the restriction endonuclease Bcg I has been constructed. It contains 2.5×10 6 independent clones, representing several times the total human genome which should contain about 400 000 such fragments. It is proposed to m...

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Bibliographic Details
Published in:Gene 1998-06, Vol.213 (1), p.17-22
Main Authors: Saupe, S, Bernard, P, Laurent-Brun, E, Derancourt, J, Roizès, G
Format: Article
Language:English
Subjects:
Cloning, Molecular
CpG Islands
Deoxyribonucleases, Type II Site-Specific
DNA, Complementary - genetics
DNA, Recombinant
Gene Library
Genome, Human
Humans
Plasmids
Substrate Specificity
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Summary:A library made up of 36 bp DNA fragments generated by digestion of human DNA with the restriction endonuclease Bcg I has been constructed. It contains 2.5×10 6 independent clones, representing several times the total human genome which should contain about 400 000 such fragments. It is proposed to make use of these BcgI fragments to clone part of the coding sequences contained in the minor H3 isochore which represents 3% of the human genomic DNA and a quarter of all genes.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(98)00216-9