mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and...

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Bibliographic Details
Published in:FEBS letters 1990-08, Vol.269 (1), p.128-130
Main Authors: Slagboom, P.Eline, de Leeuw, Wiljo J.F., Vijg, Jan
Format: Article
Language:English
Subjects:
5mC, 5-methylcytosine
Aging
Animals
Biological and medical sciences
Blotting, Northern
Blotting, Southern
DNA methylation
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes
kb, kilobasepairs
Liver - physiology
Methylation
Molecular and cellular biology
Molecular genetics
mRNA
OTC, ornithine transcarbamoylase (EC 2.1.3.3)
Rats
RNA, Messenger - genetics
TAT, tyrosine aminotransferase (EC 2.6.1.5)
Tyrosine aminotransferase
Tyrosine Transaminase - genetics
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Summary:We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)81136-C