Detection of pathogenic Yersinia enterocolitica using a digoxigenin labelled probe targeting the yst gene

A 145 base pair digoxigenin‐d‐UTP‐labelled probe, specific for pathogenic Yersinia enterocolitica heat‐stable enterotoxin yst gene, was prepared by PCR. The probe was used in DNA‐DNA colony hybridization and dot‐blot hybridization assays. The specificity of the probe was confirmed using 52 strains r...

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Bibliographic Details
Published in:Journal of applied microbiology 1998-02, Vol.84 (2), p.285-292
Main Authors: DURISIN, M. D, IBRAHIM, A, GRIFFITHS, M. W
Format: Article
Language:English
Subjects:
Bacterial Toxins - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Digoxigenin
DNA Probes
DNA, Ribosomal - genetics
Enterotoxins - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Microbiology
RNA, Ribosomal, 16S - genetics
Sensitivity and Specificity
Yersinia enterocolitica - classification
Yersinia enterocolitica - genetics
Yersinia enterocolitica - pathogenicity
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Summary:A 145 base pair digoxigenin‐d‐UTP‐labelled probe, specific for pathogenic Yersinia enterocolitica heat‐stable enterotoxin yst gene, was prepared by PCR. The probe was used in DNA‐DNA colony hybridization and dot‐blot hybridization assays. The specificity of the probe was confirmed using 52 strains representing all Yersinia spp., except Y. pestis. Out of a total of 25 Y. enterocolitica strains screened, the probe correctly identified all 18 pathogenic strains. Among the other Yersinia spp. screened, only one strain of Y. kristensenii was positively detected by the yst probe but could be differentiated by its weak signal response as compared with that obtained by pathogenic strains of Y. enterocolitica.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.1998.00344.x