Interferon α activates the tyrosine kinase Lyn in haemopoietic cells

We investigated whether the src‐family tyrosine kinase Lyn is involved in the generation of interferon α (IFNα) signals in haemopoietic cells. In vitro kinase assays using IFNα‐sensitive cells of B‐cell origin demonstrated the presence of IFNα‐dependent kinase activity in anti‐Lyn immunoprecipitates...

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Bibliographic Details
Published in:British journal of haematology 1998-06, Vol.101 (3), p.446-449
Main Authors: Uddin, Shahab, Grumbach, Isabella M., YI, Taolin, Colamonici, Oscar R., Platanias, Leonidas C.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Western
Cell Communication
Cell Line
Fundamental and applied biological sciences. Psychology
Hematology
Hematopoietic Stem Cells - enzymology
Humans
interferon
Interferon-alpha - pharmacology
Protein-Tyrosine Kinases - metabolism
Signal Transduction
tyrosine kinases
Vertebrates: blood, hematopoietic organs, reticuloendothelial system
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Summary:We investigated whether the src‐family tyrosine kinase Lyn is involved in the generation of interferon α (IFNα) signals in haemopoietic cells. In vitro kinase assays using IFNα‐sensitive cells of B‐cell origin demonstrated the presence of IFNα‐dependent kinase activity in anti‐Lyn immunoprecipitates. Further studies demonstrated that Lyn associates via its src homology 2 (SH2) domain with the Janus family tyrosine kinase Tyk‐2. This interaction was IFNα‐dependent and involved direct binding of the SH2 domain of Lyn to the IFNα‐activated form of Tyk‐2. Thus, during binding of IFNα to its receptor in malignant haemopoietic cells, Lyn is engaged in an IFNα‐signalling pathway, probably downstream of Tyk‐2.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1998.00731.x