EXPERIMENTAL APPROACHES FOR OBSERVING HOMOLOGOUS DESENSITISATION AND THEIR PITFALLS

Whereas molecular mechanisms of cell desensitisation have been discussed at length in the literature, little organised information on the methods for studying desensitisation of cellular responses has been published. In this article, three commonly utilised protocols for studying homologous desensit...

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Bibliographic Details
Published in:Pharmacological research 1998-04, Vol.37 (4), p.285-288
Main Authors: MODEL, MICHAEL A., OMANN, GENEVA M.
Format: Article
Language:English
Subjects:
Animals
Cell Physiological Phenomena
Cell Separation
desensitisation, experimental design, neutrophils, oxidants,N-formyl peptides
Humans
In Vitro Techniques
Signal Transduction - physiology
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Summary:Whereas molecular mechanisms of cell desensitisation have been discussed at length in the literature, little organised information on the methods for studying desensitisation of cellular responses has been published. In this article, three commonly utilised protocols for studying homologous desensitisation of cellular responses are evaluated. These are (1) observation of attenuation of a response after an initial stimulus-induced activation, (2) repeated stimulation of cells after washing away the previous stimulus, and (3) repeated stimulation without a wash step. Advantages and limitations of each protocol are discussed and data is presented demonstrating some of the properties of the protocols.
ISSN:1043-6618
1096-1186
DOI:10.1006/phrs.1998.0294