Characterization and expression of a stage specific antigen by monoclonal antibody TRA 54 in testicular germ cells

To study the mechanism of spermatogenesis, we have isolated many monoclonal antibodies (mAb) which recognize specific steps of mouse germ cell differentiation and then have evaluated the specific expression and characterization of antigenic molecules using immunohistochemistry and Western blotting....

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Bibliographic Details
Published in:International journal of andrology 1998-04, Vol.21 (1), p.34-40
Main Authors: PEREIRA, L. A. V. D, TANAKA, H, NAGATA, Y, SAWADA, K, MORI, H, CHIMELLI, L. M. C, NISHIMUNE, Y
Format: Article
Language:English
Subjects:
acrosome
Animals
Antibodies, Monoclonal - immunology
Antigens, Surface - biosynthesis
Antigens, Surface - immunology
Biological and medical sciences
Blotting, Western
chromatoid body
Fundamental and applied biological sciences. Psychology
Immunoenzyme Techniques
mAb
Male
Mammalian male genital system
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Morphology. Physiology
Rats
Rats, Inbred F344
spermatid
Spermatozoa - growth & development
Spermatozoa - immunology
testicular germ cell
Testis - growth & development
Testis - immunology
Vertebrates: reproduction
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Summary:To study the mechanism of spermatogenesis, we have isolated many monoclonal antibodies (mAb) which recognize specific steps of mouse germ cell differentiation and then have evaluated the specific expression and characterization of antigenic molecules using immunohistochemistry and Western blotting. Monoclonal antibody TRA 54 recognized specific organelles in germ cell cytoplasm from spermatocytes to spermatids; that is, a large granule was stained in mid–late pachytene, diplotene and secondary spermatocytes and in round spermatids at stage I while the acrosome of spermatids at steps 2–3 to step 12 were also positive. Thereafter, the antigens disappeared from spermatids at more advanced stages of differentiation. Western blots using TRA 54 revealed broad bands with approximate molecular weights of >200, 190 and 85 kDa in the testis. The expression of these antigens during testicular germ cell development should be of interest in relation to the biogenesis of organelles such as the chromatoid body and acrosome and will be a useful stage‐specific molecular marker for the study of spermatogenesis.
ISSN:0105-6263
1365-2605
DOI:10.1046/j.1365-2605.1998.00086.x