High-temperature inducible cell-free transcription and replication of double-stranded RNAs within the parasitic protozoan Cryptosporidium parvum

Sporozoites of the protozoan parasite, Cryptosporidium parvum, were found to contain free, full-size plus strands transcribed from two extrachromosomal, cytoplasmic, virus-like double-stranded RNAs (dsRNAs). Cell-free transcription and replication of both dsRNAs were observed in crude sporozoite lys...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1998-06, Vol.245 (2), p.331-337
Main Authors: Khramtsov, N V, Upton, S J
Format: Article
Language:English
Subjects:
Animals
Cell-Free System
Cryptosporidium parvum
Cryptosporidium parvum - genetics
Cryptosporidium parvum - virology
RNA, Viral - genetics
Temperature
Transcription, Genetic
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Summary:Sporozoites of the protozoan parasite, Cryptosporidium parvum, were found to contain free, full-size plus strands transcribed from two extrachromosomal, cytoplasmic, virus-like double-stranded RNAs (dsRNAs). Cell-free transcription and replication of both dsRNAs were observed in crude sporozoite lysates. RNA polymerase activity was found to be dependent upon addition of Mg2+ or Mn2+, as well as the four ribonucleoside triphosphates, and was insensitive to inhibitors of cellular DNA-dependent RNA polymerase. Semiconservative transcription of the dsRNAs (plus strand synthesis) was observed at a wide range of temperatures, with an optimum of 50 degrees C. In contrast, replication (minus strand synthesis) was detected only at 50 and 60 degrees C.
ISSN:0042-6822
DOI:10.1006/viro.1998.9181