Expression of the gene encoding subunit II of yeast QH2: cytochrome c oxidoreductase is regulated by multiple factors

In Saccharomyces cerevisiae, the COR2 gene codes for the 40 kDa subunit II of the QH2: cytochrome c oxidoreductase, an enzyme of the mitochondrial respiratory chain. Regions in the 5' flank of this gene important for regulated expression were identified by assaying beta-galactosidase activities...

Full description

Saved in:
Bibliographic Details
Published in:Current genetics 1990-06, Vol.17 (6), p.459-464
Main Authors: Dorsman, J.C, Grivell, L.A
Format: Article
Language:English
Subjects:
Base Sequence
Binding, Competitive
Biological and medical sciences
cytochrome-c oxidase
DNA, Fungal - genetics
DNA-Binding Proteins - metabolism
Electron Transport Complex III - genetics
Fundamental and applied biological sciences. Psychology
Fungal Proteins - metabolism
Gene expression
Gene Expression Regulation, Fungal
genes
mitochondria
mitochondrial biogenesis
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutation
Oxygen Consumption
regulation
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
transcription (genetics)
Transcription, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In Saccharomyces cerevisiae, the COR2 gene codes for the 40 kDa subunit II of the QH2: cytochrome c oxidoreductase, an enzyme of the mitochondrial respiratory chain. Regions in the 5' flank of this gene important for regulated expression were identified by assaying beta-galactosidase activities in cells carrying different COR2-lacZ fusion genes. Sequences downstream of position -201 relative to the translational initiation codon are sufficient to confer regulation by carbon source, whereas sequences downstream of position -153 do not give rise to significant expression. A binding site for the abundant general transcription factor GFI is present in the region between -201 and -153 just upstream from sequences which resemble the consensus DNA recognition sequence of the regulatory protein complex HAP2/HAP3: 5'-TNATTGGT-3'. By quantitating RNA levels and assaying beta-galactosidase activities we show that synthesis of COR2, which is not a hemoprotein, is regulated by HAP1, HAP2/HAP3 and heme.
ISSN:0172-8083
1432-0983
DOI:10.1007/bf00313072