Identification and Cloning of an Orphan G Protein-Coupled Receptor of the Glycoprotein Hormone Receptor Subfamily

• Published in: Biochemical and biophysical research communications 1998-06, Vol.247 (2), p.266-270
• Main Authors: McDonald, Terry, Wang, Ruiping, Bailey, Wendy, Xie, Guochun, Chen, Fang, Caskey, C.Thomas, Liu, Qingyun
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Chromosome Mapping; Chromosomes, Human, Pair 12 - genetics; Cloning, Molecular; DNA, Complementary - genetics; Female; Gene Expression; GTP-Binding Proteins - metabolism; Humans; Ligands; Molecular Sequence Data; Pregnancy; Receptors, Cell Surface - classification; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Sequence Homology, Amino Acid; Tissue Distribution
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1998.8774

Mining of the EST database identified a human EST that was predicted to encode a novel member of the glycoprotein hormone receptor subfamily. Based on the sequence information, the full-length coding region of this gene was isolated and sequenced. This gene, designated HG38, is predicted to encode a polypeptide of 907 amino acid residues with a putative signal peptide sequence at its very N-terminus. HG38 is most closely related to members of the glycoprotein hormone receptor subfamily with ∼35% overall identity at the protein sequence level. As with the glycoprotein hormone receptors, HG38 contains a long extracellular domain with a total of 16 leucine-rich repeats. Northern blot analysis showed that HG38 was expressed in skeletal muscle, placenta, spinal cord, and various regions of the brain. Radiation hybrid mapping placed HG38 into human chromosome 12q22-23. HG38 is most likely to be a receptor for a novel class of glycoprotein ligands.