Development of a specific radioimmunoassay for vitamin B12 and its application in the diagnosis of cobalt deficiency in sheep

A radioimmunoassay (RIA) for vitamin B12 is described. Antisera were raised in rabbits using a conjugate between 5'-O-succinyl cyanocobalamin and chicken serum albumin. The antibody is largely specific for the beta (upper) face of the vitamin B12 molecule. The RIA can detect vitamin B12 down to...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary research communications 1990-01, Vol.14 (4), p.255-265
Main Authors: Kennedy, D.G. (Veterinary Research Labs., Belfast (UK)), O'Harte, F.P.M, Blanchflower, W.J, Rice, D.A
Format: Article
Language:English
Subjects:
Animals
ANTIBODIES
Antibody Specificity
ANTICORPS
ANTICUERPOS
CARENCE EN VITAMINE
COBALT
Cobalt - deficiency
COBALTO
Cross Reactions
DEFICIENCIA DE VITAMINAS
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
Immune Sera - immunology
IMMUNE SERUM
IMMUNSERUM
OVIN
OVINOS
Predictive Value of Tests
RADIOIMMUNOASSAY
SHEEP
Sheep Diseases - diagnosis
SUERO INMUNE
TECHNIQUE RADIOIMMUNOLOGIQUE
TECNICAS RADIOINMUNOLOGICAS
Vitamin B 12 - blood
Vitamin B 12 - immunology
VITAMIN B12
VITAMIN DEFICIENCIES
VITAMINA B12
VITAMINE B12
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A radioimmunoassay (RIA) for vitamin B12 is described. Antisera were raised in rabbits using a conjugate between 5'-O-succinyl cyanocobalamin and chicken serum albumin. The antibody is largely specific for the beta (upper) face of the vitamin B12 molecule. The RIA can detect vitamin B12 down to concentrations of 37 pmol/L and correlated well (r = 0.980, p less than 0.001) with a commercial radioassay. The use of this RIA, together with an assay for plasma methylmalonic acid, in the diagnosis of cobalt/vitamin B12 deficiency in sheep is described.
ISSN:0165-7380
1573-7446
DOI:10.1007/BF00350708