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Frequency of Clonal B Lymphocytes in Chronic Myelogenous Leukemia Evaluated by Fluorescence In Situ Hybridization

The demonstration of the Philadelphia (Ph) chromosome in B lymphocytes from patients with chronic myelogenous leukemia (CML) has provided evidence that the disorder originates in a pluripotent progenitor cell. Divergent results, however, exist as to the degree of contribution of clonally derived cel...

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Bibliographic Details
Published in:Cancer genetics and cytogenetics 1998-07, Vol.104 (1), p.45-47
Main Authors: Al-Amin, Amir, Lennartz, Klaus, Runde, Volker, Seeber, Siegfried, Opalka, Bertram, Kloke, Otto
Format: Article
Language:English
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Summary:The demonstration of the Philadelphia (Ph) chromosome in B lymphocytes from patients with chronic myelogenous leukemia (CML) has provided evidence that the disorder originates in a pluripotent progenitor cell. Divergent results, however, exist as to the degree of contribution of clonally derived cells to the B-cell compartment. To address this issue, B lymphocytes were selected from the blood of seven patients in the chronic phase of Ph-positive CML and were examined with dual-color fluoresence in situ hybridization for the presence of the Ph translocation. The purity of the B-cell preparations ranged from 88% to 97% (mean 93%). The Ph translocation was detected in 22–34% (mean, 27%) of the sorted B cells. There was no evidence that the duration of the disease affects the ratio of Ph-positive and -negative B cells. In summary, clonally derived circulating B lymphocytes were present in all patients studied but made only minor contribution to this compartment.
ISSN:0165-4608
1873-4456
DOI:10.1016/S0165-4608(97)00435-4