DNA optical sensor: a rapid method for the detection of DNA hybridization

A DNA optical sensor system is proposed based on the combination of sandwich solution hybridization, magnetic bead capture, flow injection and chemiluminescence for rapid detection of DNA hybridization. Bacterial alkaline phosphatase ( phoA) gene and Hepatitis B virus (HBV) DNa were used as target D...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1998-03, Vol.13 (3), p.451-458
Main Authors: Chen, Xiao, Zhang, Xian-En, Chai, Yi-Quan, Hu, Wei-Ping, Zhang, Zhi-Phing, Zhang, Xiao-Mei, Cass, Anthony E.G.
Format: Article
Language:English
Subjects:
alkaline phosphatase
Biological and medical sciences
Biosensing Techniques
biosensor
Biosensors
Biotechnology
biotin
chemiluminesence
DNA - analysis
DNA hybridization
flow injection
Flow Injection Analysis
Fundamental and applied biological sciences. Psychology
HBV
Immunomagnetic Separation
Luminescent Measurements
magnetic bead
Methods. Procedures. Technologies
Nucleic Acid Hybridization
Optics and Photonics
phoA
Sensitivity and Specificity
Signal Processing, Computer-Assisted
streptavidin
Time Factors
Various methods and equipments
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Summary:A DNA optical sensor system is proposed based on the combination of sandwich solution hybridization, magnetic bead capture, flow injection and chemiluminescence for rapid detection of DNA hybridization. Bacterial alkaline phosphatase ( phoA) gene and Hepatitis B virus (HBV) DNa were used as target DNA. A biotinylated DNA probe was used to capture the target gene onto the streptavidin-coated magnetic beads and a calf intestine alkaline phosphatase (CAP)-labelled DNA probe was used for subsequent enzymatic chemiluminescence detection. The detection cycle was less than 30 min, excluding the DNA hybridization time, which was about 100 min. Both the phoA gene and HBV DNA could be detected at picogramme or femtomole level. No response signal was obtained when target DNA did not exist in the sample. Successive sample detection could be made by removing the magnetic field and a washing step.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(97)00095-X