Cryopreservation of blastocysts using a modification of a simplified freezing protocol with a one step removal of cryoprotectant successfully used previously to freeze 2 pronuclear or multi-cell embryos

To describe a modification of a simplified freezing protocol for the cryopreservation of blastocysts. 1.5 M glycerol was substituted as a cryoprotectant instead of propanediol. There was a survival rate of 59.1% (13/22) with three live deliveries in seven transfers (42.9% per transfer). The implanta...

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Bibliographic Details
Published in:Clinical and experimental obstetrics & gynecology 2010, Vol.37 (2), p.99-99
Main Authors: Summers-Chase, D, Check, J H, Horwath, D, Yuan, W, Swenson, K, Press, M
Format: Article
Language:English
Subjects:
Blastocyst
Cryopreservation - methods
Cryoprotective Agents
Humans
Online Access:Get full text
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Summary:To describe a modification of a simplified freezing protocol for the cryopreservation of blastocysts. 1.5 M glycerol was substituted as a cryoprotectant instead of propanediol. There was a survival rate of 59.1% (13/22) with three live deliveries in seven transfers (42.9% per transfer). The implantation rate was 28.6% (4/14). This is the first description of a new technique for freezing blastocysts. A larger series is needed to determine if the good pregnancy rates will continue.
ISSN:0390-6663