Effects of Tumor Necrosis Factor α and Interferon γ on the Viability and mRNA Expression of TNF Receptor Type I in Endothelial Cells from the Bovine Corpus Luteum

The corpus luteum (CL) is mainly composed of luteal steroidogenic cells (LSCs) and luteal endothelial cells (LECs). Cell death of LSCs and LECs is essential for structural luteolysis. Therefore, it is important to understand the mechanisms regulating cell death in both types of luteal cells. We prev...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2010, Vol.56(5), pp.515-519
Main Authors: HOJO, Takuo, ODA, Akihiro, LEE, Seung-Hyung, ACOSTA, Tomas J., OKUDA, Kiyoshi
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Bovine
Cattle
Cell death
Cell Survival - drug effects
Cells, Cultured
Corpus luteum
Corpus Luteum - cytology
Corpus Luteum - drug effects
Corpus Luteum - physiology
DNA Fragmentation - drug effects
Dose-Response Relationship, Drug
Drug Synergism
Endothelial Cells - cytology
Endothelial Cells - drug effects
Endothelial Cells - physiology
Female
Gene Expression - drug effects
Interferon-gamma - metabolism
Interferon-gamma - pharmacology
Luteal endothelial cell
Luteolysis
Luteolysis - drug effects
Luteolysis - physiology
Receptors, Tumor Necrosis Factor, Type I - genetics
RNA, Messenger - metabolism
Tumor Necrosis Factor-alpha - metabolism
Tumor Necrosis Factor-alpha - pharmacology
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Summary:The corpus luteum (CL) is mainly composed of luteal steroidogenic cells (LSCs) and luteal endothelial cells (LECs). Cell death of LSCs and LECs is essential for structural luteolysis. Therefore, it is important to understand the mechanisms regulating cell death in both types of luteal cells. We previously reported that a treatment combining tumor necrosis factor α (TNF) and interferon γ (IFNG) induced cell death in LSCs and that LECs express TNF receptor type I (TNFRI). To investigate the mechanism of cell death in LECs, in the present study we determined the effects of the same cytokines on cell viability and TNFRI mRNA expression in cultured LECs. To induce cell death in LECs, LECs were treated with TNF or IFNG (0, 0.05, 0.5, 1.0, 2.5 nM) and a combination of TNF (0.5 nM) and IFNG (0.5 nM) for 24 h. The viability of LECs was reduced by a single treatment with TNF or IFNG dose-dependently (P
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.10-056T