Nuclear Magnetic Resonance (NMR) Study of the Effect of Cisplatin on the Metabolic Profile of MG-63 Osteosarcoma Cells

In the present study, 1H HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enab...

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Bibliographic Details
Published in:Journal of proteome research 2010-11, Vol.9 (11), p.5877-5886
Main Authors: Duarte, Iola F, Lamego, Inês, Marques, Joana, Marques, M. Paula M, Blaise, Benjamin J, Gil, Ana M
Format: Article
Language:English
Subjects:
Alcohols
Amino Acids
Choline
Cisplatin - pharmacology
Humans
Kinetics
Lipids
Magnetic Resonance Spectroscopy - methods
Metabolome - drug effects
Osteosarcoma - drug therapy
Osteosarcoma - metabolism
Osteosarcoma - pathology
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Summary:In the present study, 1H HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr100635n