Adhesion of Human B Cells to Germinal Centers in Vitro Involves VLA-4 and INCAM-110

Human B lymphocytes localize and differentiate within the microenvironment of lymphoid germinal centers. A frozen section binding assay was developed for the identification of those molecules involved in the adhesive interactions between B cells and lymphoid follicles. Activated human B cells and B...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1990-08, Vol.249 (4972), p.1030-1033
Main Authors: Freedman, Arnold S., Munro, J. Michael, Rice, G. Edgar, Bevilacqua, Michael P., Morimoto, Chikao, McIntyre, Bradley W., Rhynhart, Kurt, Pober, Jordan S., Nadler, Lee M.
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Monoclonal
Antigens, CD - analysis
B cells
B lymphocytes
B-Lymphocytes - cytology
B-Lymphocytes - immunology
B-Lymphocytes - ultrastructure
Biological and medical sciences
Butchering
Cell Adhesion
Cell Adhesion Molecules - immunology
Cell lines
Cells, Cultured
Follicles
Follicular dendritic cells
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Immunology
Lymphocytes
Lymphoid organs: ontogeny, organization, homing phenomenon
Lymphoid tissue
Molecular aspects
Molecular biology
Molecules
Palatine Tonsil - cytology
Palatine Tonsil - immunology
Physiological aspects
Receptors, Very Late Antigen - immunology
Spleen - immunology
Vascular Cell Adhesion Molecule-1
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Summary:Human B lymphocytes localize and differentiate within the microenvironment of lymphoid germinal centers. A frozen section binding assay was developed for the identification of those molecules involved in the adhesive interactions between B cells and lymphoid follicles. Activated human B cells and B cell lines were found to selectively adhere to germinal centers. The VLA-4 molecule on the lymphocyte and the adhesion molecule INCAM-110, expressed on follicular dendritic cells, supported this interaction. This cellular interaction model can be used for the study of how B cells differentiate.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1697696