Using molecular techniques to identify new microbial biocatalysts

Evolution has favoured microorganisms that produce efficient enzymes with substrate-adapted biocatalytic activities. Progress in molecular techniques, especially expression cloning, molecular screening, protein engineering and in vivo and in vitro shuffling, have paved the way for greater speed and...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 1998-06, Vol.16 (6), p.265-272
Main Authors: Dalbøge, Henrik, Lange, Lene
Format: Article
Language:English
Subjects:
Amino Acid Sequence
artificial evolution
Bacteria - enzymology
Biological and medical sciences
Biotechnology
Cloning, Molecular
Enzymes - isolation & purification
expression cloning
Fundamental and applied biological sciences. Psychology
Fungi - enzymology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
microbial enzymes
Miscellaneous
molecular screening
Molecular Sequence Data
Protein Engineering
Synthetic digonucleotides and genes. Sequencing
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Summary:Evolution has favoured microorganisms that produce efficient enzymes with substrate-adapted biocatalytic activities. Progress in molecular techniques, especially expression cloning, molecular screening, protein engineering and in vivo and in vitro shuffling, have paved the way for greater speed and accuracy in cloning enzyme genes from microorganisms and generating versions with improved properties. Recently, two new approaches have been added: screening directly from uncultivated microorganisms and generating additional hits by database mining using bioinformatic tools.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(98)01202-5