Variable Bcl-2 phenotype in benign and malignant lesions of urothelium

We examined Bcl-2, Bax and p53 expression in transitional epithelium, benign lesions derived from transitional epithelium (Brunn's nests and inverted papillomas) and transitional cell cancer (TCC) of the upper urinary tract and urinary bladder using immunostaining of cryostat sections. We also...

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Bibliographic Details
Published in:Cancer letters 1998-06, Vol.128 (1), p.87-92
Main Authors: Bilim, Vladimir N, Tomita, Yoshihiko, Kawasaki, Takashi, Takeda, Masayuki, Takahashi, Kota
Format: Article
Language:English
Subjects:
Adult
Aged
Apoptosis
Bax
Bcl-2
bcl-2-Associated X Protein
Biological and medical sciences
Carcinoma, Transitional Cell - genetics
Carcinoma, Transitional Cell - metabolism
Female
Gene Expression
Humans
Male
Medical sciences
Middle Aged
Nephrology. Urinary tract diseases
p53
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-bcl-2 - metabolism
Transitional cell cancer
Tumor Suppressor Protein p53 - metabolism
Tumors of the urinary system
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - metabolism
Urinary tract. Prostate gland
Urogenital Neoplasms - metabolism
Urothelium - metabolism
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Summary:We examined Bcl-2, Bax and p53 expression in transitional epithelium, benign lesions derived from transitional epithelium (Brunn's nests and inverted papillomas) and transitional cell cancer (TCC) of the upper urinary tract and urinary bladder using immunostaining of cryostat sections. We also performed Western blot analysis of normal urothelium and TCCs for Bcl-2 and p53. Immunohistochemical staining showed that Bcl-2 was expressed only on basal layer cells, whereas Bax expression was restricted to superficial layers in normal transitional epithelium. Benign lesions of the urinary bladder (Brunn's nests and inverted papillomas) showed strong immunoreactivity to Bcl-2. Taken together, 16 (17%) TCCs were positive for Bcl-2, 62 (64.6%) TCCs were positive for Bax and 28 (29%) TCCs were positive for p53. The expression of Bcl-2 on TCC had a statistical correlation with tumor stage, histopathologic grade and p53 protein accumulation. The results suggest that although Bcl-2 overexpression is detected in normal urothelium and benign lesions of the urinary bladder, it might also contribute to the high grade tumor malignancy of TCC.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(98)00055-X