Loading…
Cellular Immune Responses to Four Doses of Percutaneous Bacille Calmette-Guérin in Healthy Adults
To explore the hypothesis that low-dose immunization might induce preferential Th1 cell immunity, 76 adults were vaccinated with one of four doses of bacille Calmette-Guérin (BCG): The doses contained very low (1.6 × 105 cfu), low (3.2 × 106 cfu), standard (1.6 × 108 cfu), or high (3.2 × 108 cfu) le...
Saved in:
Published in: | The Journal of infectious diseases 1998-07, Vol.178 (1), p.138-146 |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | To explore the hypothesis that low-dose immunization might induce preferential Th1 cell immunity, 76 adults were vaccinated with one of four doses of bacille Calmette-Guérin (BCG): The doses contained very low (1.6 × 105 cfu), low (3.2 × 106 cfu), standard (1.6 × 108 cfu), or high (3.2 × 108 cfu) levels of BCG. Delayed-type hypersensitivity responses occurred 8 weeks after vaccination in 10% of persons given very low or low doses of BCG, compared with 95% and 100% of persons given standard or high doses, respectively. Lymphoproliferative responses, which were increased only for high-dose vaccinees, peaked 2 weeks after vaccination and were directed chiefly against Mycobacterium tuberculosis-secreted proteins, particularly the antigen 85 complex. Significant increases in mycobacteria-specific interferon-γ expression were present 16 weeks after vaccination only for persons given standard or high doses of BCG. Percutaneous BCG appears capable of inducing a temporary Th1-like immune response, but standard or higher dosages are required. |
---|---|
ISSN: | 0022-1899 1537-6613 |
DOI: | 10.1086/515614 |