Mixer, a Homeobox Gene Required for Endoderm Development

An expression cloning strategy in Xenopus laevis was used to isolate a homeobox-containing gene, Mixer, that can cause embryonic cells to form endoderm. Mixer transcripts are found specifically in the prospective endoderm of gastrula, which coincides with the time and place that endodermal cells bec...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1998-07, Vol.281 (5373), p.91-96
Main Authors: Henry, Gilbert L., Melton, Douglas A.
Format: Article
Language:English
Subjects:
Anatomy
Animal embryology
Animals
Antisense elements
Biological and medical sciences
Blastocyst - cytology
Blastocyst - physiology
Cell Lineage
Cell lines
Cells
Classical genetics, quantitative genetics, hybrids
Cloning
Cloning, Molecular
DNA-Binding Proteins
Eggs
Embryogenesis
Embryology
Embryology: invertebrates and vertebrates. Teratology
Embryonic Induction
Embryos
Endoderm
Endoderm - cytology
Endoderm - physiology
Freshwater
Fundamental and applied biological sciences. Psychology
Gastrula - cytology
Gastrula - physiology
Gene Expression Regulation, Developmental
Genes
Genes, Homeobox
Genetic aspects
Genetics of eukaryotes. Biological and molecular evolution
High Mobility Group Proteins
Homeobox genes
Homeodomain Proteins - genetics
Homeotic genes
In Situ Hybridization
Individualized Instruction
Mesoderm
Mesoderm - cytology
Mesoderm - physiology
Messenger RNA
Molecular Sequence Data
Organogenesis. Fetal development
Organogenesis. Physiological fonctions
Proteins - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Screening Tests
SOXF Transcription Factors
Transcription Factors - genetics
Transcription Factors - physiology
Transcripts (Written Records)
Vertebrata
Xenopus laevis
Xenopus Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An expression cloning strategy in Xenopus laevis was used to isolate a homeobox-containing gene, Mixer, that can cause embryonic cells to form endoderm. Mixer transcripts are found specifically in the prospective endoderm of gastrula, which coincides with the time and place that endodermal cells become histologically distinct and irreversibly determined. Loss-of-function studies with a dominant inhibitory mutant demonstrate that Mixer activity is required for endoderm development. In particular, the expression of Sox17α and Sox17β, two previously identified endodermal determinants, require Mixer function. Together, these data suggest that Mixer is an embryonic transcription factor involved in specifying the endodermal germ layer.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.281.5373.91