Predicted and observed alleles of Plasmodium falciparum merozoite surface protein-1 (MSP-1), a potential malaria vaccine antigen

The 19-kDa antigenic domain of Plasmodium falciparum merozoite surface protein (MSP)-1 is a potential malaria vaccine candidate. Based on the amino acid substitution, four known alleles, E-TSR (PNG-MAD20 type), E-KNG (Uganda-PA type), Q-KNG (Wellcome type), and Q-TSR (Indo type) of this domain have...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1998-05, Vol.92 (2), p.241-252
Main Authors: Qari, Shoukat H, Shi, Ya-Ping, Goldman, Ira F, Nahlen, Bernard L, Tibayrenc, Michel, Lal, Altaf A
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Animals
Base Sequence
Cross-Sectional Studies
DNA, Protozoan
Humans
Infant
Kenya - epidemiology
Longitudinal Studies
Malaria
Malaria Vaccines - genetics
Malaria Vaccines - immunology
Malaria, Falciparum - epidemiology
Malaria, Falciparum - parasitology
Merozoite Surface Protein 1
Molecular Sequence Data
Mutation
Parasite
Plasmodium falciparum
Plasmodium falciparum - genetics
Plasmodium falciparum - immunology
Polymerase Chain Reaction
Protein Precursors - genetics
Protein Precursors - immunology
Protein Precursors - isolation & purification
Protozoan Proteins - genetics
Protozoan Proteins - immunology
Protozoan Proteins - isolation & purification
Sequence Alignment
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Summary:The 19-kDa antigenic domain of Plasmodium falciparum merozoite surface protein (MSP)-1 is a potential malaria vaccine candidate. Based on the amino acid substitution, four known alleles, E-TSR (PNG-MAD20 type), E-KNG (Uganda-PA type), Q-KNG (Wellcome type), and Q-TSR (Indo type) of this domain have been identified. Using single or double crossover recombinational events, we predicted the existence of additional alleles of this antigen. The presence of the predicted alleles was determined in parasite isolates from western Kenya, by undertaking a cross-sectional and a longitudinal study. Of the ten predicted alleles, we have revealed the presence of three new alleles: E-KSG-L (Kenya-1 type); E-KSR-L (Kenya-2 type); and E-KNG-F (Kenya-3 type). The results of this study suggest that it may be possible to predict the complexity of the genetic makeup of natural parasite populations.
ISSN:0166-6851
1872-9428
DOI:10.1016/S0166-6851(98)00010-3