Transcription analysis of porcine circovirus (PCV)

This study focuses on gene expression of porcine circovirus (PCV) in order to identify viral genes and their corresponding mRNA transcripts. By northern blot analysis, the existence of three mRNAs could be demonstrated. Two mRNAs are encoded by the viral (-)-strand and one is encoded by the viral (+...

Full description

Saved in:
Bibliographic Details
Published in:Virus genes 1998, Vol.16 (3), p.267-276
Main Authors: Mankertz, J. (Robert Koch-Inst., Berlin (Germany). Fachbereich Gentechnik und Genetik), Buhk, H.J, Blaess, G, Mankertz, A
Format: Article
Language:English
Subjects:
ADN
Animals
ARN
Base Sequence
Cell Line
CERDO
CIRCOVIRIDAE
Circovirus - genetics
DNA
DNA, Viral
EXPRESION GENICA
EXPRESSION DES GENES
GENE EXPRESSION
Molecular Sequence Data
Open Reading Frames
Peptide Chain Initiation, Translational
PORCIN
Regulatory Sequences, Nucleic Acid
RNA
RNA Probes
RNA Splicing
RNA, Viral - analysis
SWINE
TRANSCRIPCION
TRANSCRIPTION
Transcription, Genetic
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study focuses on gene expression of porcine circovirus (PCV) in order to identify viral genes and their corresponding mRNA transcripts. By northern blot analysis, the existence of three mRNAs could be demonstrated. Two mRNAs are encoded by the viral (-)-strand and one is encoded by the viral (+)-strand. The (+)-strand encoded mRNA transcript is 990 nucleotides (nt) long and corresponds to the open reading frame (ORF) 1, as shown by S1 mapping. The start point of this transcript is located at pos. 1238, as determined by primer extension analysis and rapid amplification of cDNA ends (RACE). The transcript is spliced as shown by direct reverse sequencing and RACE. It contains an untranslated "leader"-sequence 119 nt in size (pos. 1238 to 1120) which is joined to exon 2 of the ORF 1 transcript at pos. 737. The transcriptional regulatory elements have been identified functionally by CAT assays. They are located within a 258 base points (bp) fragment (pos. 1168 to 1425).
ISSN:0920-8569
1572-994X
DOI:10.1023/A:1008022521329