Comparison of a novel micro-assay for insulin autoantibodies with the conventional radiobinding assay

Measurement of insulin autoantibodies (IAA) with a novel micro radiobinding assay which requires only 20 microl of serum was compared with that in a conventional radiobinding assay which uses 600 microl of serum. IAA were measured with both assays in samples from 94 new onset insulin-dependent diabe...

Full description

Saved in:
Bibliographic Details
Published in:Diabetologia 1998-06, Vol.41 (6), p.681-683
Main Authors: NASERKE, H. E, DOZIO, N, ZIEGLER, A.-G, BONIFACIO, E
Format: Article
Language:English
Subjects:
Adolescent
Adult
Autoantibodies - blood
Biological and medical sciences
Child
Child, Preschool
Diabetes Mellitus, Type 1 - blood
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Female
Humans
Infant
Insulin - immunology
Male
Medical sciences
Microchemistry - methods
Middle Aged
Radioligand Assay - methods
Sensitivity and Specificity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Measurement of insulin autoantibodies (IAA) with a novel micro radiobinding assay which requires only 20 microl of serum was compared with that in a conventional radiobinding assay which uses 600 microl of serum. IAA were measured with both assays in samples from 94 new onset insulin-dependent diabetes mellitus (IDDM) patients, 97 control subjects. and 48 first degree relatives of IDDM patients selected for having IAA in the conventional radiobinding assay. Overall, 227 (95%) of 239 samples tested were concordant. and IAA levels correlated well (r2 = 0.7) between the two assays. Discordant results were obtained in 7 new onset patients, 4 control subjects, and 1 first degree relative, and these had low IAA levels in the respective assays. Sensitivity and specificity in the new onset IDDM patients and control subjects were 69% and 98% for the micro radiobinding assay and 72% and 98% for the conventional radiobinding assay. The use of the micro radiobinding assay should greatly facilitate islet related antibody screening, particularly in children.
ISSN:0012-186X
1432-0428
DOI:10.1007/s001250050968