The First Caldesmon-like Protein in Higher Plants

Using anti-caldesmon polyclonal and monoclonal (raised against the N-terminal fragment of chicken gizzard caldesmon) antibodies, a plant caldesmon-like protein, 107 kDa as determined by SDS-gel electrophoresis, has been identified based on Western blotting of total extracts of Ornithogalum virenspol...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-06, Vol.247 (3), p.576-579
Main Authors: Krauze, Katarzyna, Makuch, Robert, Stȩpka, Małgorzata, Da̧browska, Renata
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Calcium - metabolism
Calmodulin - metabolism
Calmodulin-Binding Proteins - chemistry
Immunoblotting
LILIACEAE
Muscle, Skeletal - chemistry
ORNITHOGALUM VIRENS
Plant Proteins - chemistry
Pollen - chemistry
Protein Binding - physiology
PROTEINAS
PROTEINE
PROTEINS
Rabbits
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Summary:Using anti-caldesmon polyclonal and monoclonal (raised against the N-terminal fragment of chicken gizzard caldesmon) antibodies, a plant caldesmon-like protein, 107 kDa as determined by SDS-gel electrophoresis, has been identified based on Western blotting of total extracts of Ornithogalum virenspollen tubes. Biochemical investigations showed common properties of this protein with animal caldesmon—it binds to actin and, in a Ca 2+-dependent manner, to calmodulin. In contrast to animal caldesmon, this plant cell counterpart is relatively resistant to proteolysis by endogenous proteases and sensitive to heat treatment. Our results show the presence of a caldesmon-like protein in higher plants for the first time.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.8833