Simultaneous determination of melatonin–pyridoxine combination in tablets by zero-crossing derivative spectrophotometry and spectrofluorimetry

Two methods have been developed for the analysis of melatonin (M) and pyridoxine hydrochloride (PH) in combination. The first method depends on first- and second-derivative ultraviolet spectrophotometry, with the zero crossing technique of measurement. First-derivative amplitudes at 296 nm and secon...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1998-07, Vol.17 (3), p.379-386
Main Authors: Abdine, Heba H, Gazy, Azza A, Abdel-Hay, Mohamed H
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Derivative spectrophtometry
Dosage form
General pharmacology
Medical sciences
Melatonin
Melatonin - analysis
Pharmacology. Drug treatments
Pyridoxine - analysis
Pyridoxine hydrochloride
Simultaneous determination
Spectrofluorimetry
Spectrometry, Fluorescence - methods
Spectrophotometry, Ultraviolet - methods
Tablets - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two methods have been developed for the analysis of melatonin (M) and pyridoxine hydrochloride (PH) in combination. The first method depends on first- and second-derivative ultraviolet spectrophotometry, with the zero crossing technique of measurement. First-derivative amplitudes at 296 nm and second-derivative amplitudes at 294 and 322 nm are selected for the determination of M and PH, respectively. The second method is based on the native fluorescence of both M and PH, in methanol and 0.1 M hydrochloric acid, respectively, after a preliminary solvent extraction procedure. The relative standard deviation of both methods was less than 2.0%. The two methods have been successfully applied to the determination of both drugs in laboratory-prepared mixtures and in tablets
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(97)00221-5