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A rapid liquid chromatographic method for the determination of lamotrigine in plasma
A rapid, sensitive and simple high-performance liquid chromatographic (HPLC) method for the determination of lamotrigine in plasma is described. The drug was extracted from 100 μl of plasma with chloroform:isopropanol (95:5% v/v) in the presence of 100 μl of phosphate buffer (10 mM). The extract was...
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Published in: | Journal of pharmaceutical and biomedical analysis 1998-07, Vol.17 (3), p.525-531 |
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creator | Matar, K.M Nicholls, P.J Bawazir, S.A Al-Hassan, M.I Tekle, A |
description | A rapid, sensitive and simple high-performance liquid chromatographic (HPLC) method for the determination of lamotrigine in plasma is described. The drug was extracted from 100 μl of plasma with chloroform:isopropanol (95:5% v/v) in the presence of 100 μl of phosphate buffer (10 mM). The extract was evaporated and the residue was reconstituted with mobile phase and injected onto the HPLC system. The drug and the internal standard (chloramphenicol) were eluted from a Symmetry C
18 stainless steel column at ambient temperature with a mobile phase consisting of 0.01 M potassium phosphate–acetonitrile–methanol (70:20:10% v/v/v), adjusted to pH 6.7, at a flow rate of 1.3 ml min
−1 and the detector was monitored at 214 nm. Quantitation was achieved by measurement of the peak-area ratio of the drug to the internal standard and the lower limit of detection for lamotrigine in plasma was 20 ng ml
−1. The intraday precision ranged from 3.34 to 6.12% coefficient of variation (CV) and the interday precision ranged from 2.15 to 8.34% CV. The absolute and relative recoveries of lamotrigine ranged from 86.93 to 90.71% and from 95.18 to 107.13%, respectively. The method was applied in studying the pharmacokinetics of lamotrigine administered orally to rabbits. This reliable micro-method would have application in pharmacokinetic studies of lamotrigine where only small sample sizes are available, e.g. paediatric patients. |
doi_str_mv | 10.1016/S0731-7085(97)00234-3 |
format | article |
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18 stainless steel column at ambient temperature with a mobile phase consisting of 0.01 M potassium phosphate–acetonitrile–methanol (70:20:10% v/v/v), adjusted to pH 6.7, at a flow rate of 1.3 ml min
−1 and the detector was monitored at 214 nm. Quantitation was achieved by measurement of the peak-area ratio of the drug to the internal standard and the lower limit of detection for lamotrigine in plasma was 20 ng ml
−1. The intraday precision ranged from 3.34 to 6.12% coefficient of variation (CV) and the interday precision ranged from 2.15 to 8.34% CV. The absolute and relative recoveries of lamotrigine ranged from 86.93 to 90.71% and from 95.18 to 107.13%, respectively. The method was applied in studying the pharmacokinetics of lamotrigine administered orally to rabbits. This reliable micro-method would have application in pharmacokinetic studies of lamotrigine where only small sample sizes are available, e.g. paediatric patients.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(97)00234-3</identifier><identifier>PMID: 9656165</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Animals ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Dogs ; Drug Stability ; Evaluation Studies as Topic ; General pharmacology ; Humans ; Lamotrigine ; Liquid chromatography ; Male ; Medical sciences ; Pharmacokinetic studies ; Pharmacology. Drug treatments ; Plasma ; Rabbits ; Reproducibility of Results ; Sensitivity and Specificity ; Time Factors ; Triazines - blood ; Triazines - pharmacokinetics</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 1998-07, Vol.17 (3), p.525-531</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-a0e705cfb7c6e7066c7c49c20e5d7e703ac27f62436c450aa4453cc2fef54abf3</citedby><cites>FETCH-LOGICAL-c389t-a0e705cfb7c6e7066c7c49c20e5d7e703ac27f62436c450aa4453cc2fef54abf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2342384$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9656165$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matar, K.M</creatorcontrib><creatorcontrib>Nicholls, P.J</creatorcontrib><creatorcontrib>Bawazir, S.A</creatorcontrib><creatorcontrib>Al-Hassan, M.I</creatorcontrib><creatorcontrib>Tekle, A</creatorcontrib><title>A rapid liquid chromatographic method for the determination of lamotrigine in plasma</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A rapid, sensitive and simple high-performance liquid chromatographic (HPLC) method for the determination of lamotrigine in plasma is described. The drug was extracted from 100 μl of plasma with chloroform:isopropanol (95:5% v/v) in the presence of 100 μl of phosphate buffer (10 mM). The extract was evaporated and the residue was reconstituted with mobile phase and injected onto the HPLC system. The drug and the internal standard (chloramphenicol) were eluted from a Symmetry C
18 stainless steel column at ambient temperature with a mobile phase consisting of 0.01 M potassium phosphate–acetonitrile–methanol (70:20:10% v/v/v), adjusted to pH 6.7, at a flow rate of 1.3 ml min
−1 and the detector was monitored at 214 nm. Quantitation was achieved by measurement of the peak-area ratio of the drug to the internal standard and the lower limit of detection for lamotrigine in plasma was 20 ng ml
−1. The intraday precision ranged from 3.34 to 6.12% coefficient of variation (CV) and the interday precision ranged from 2.15 to 8.34% CV. The absolute and relative recoveries of lamotrigine ranged from 86.93 to 90.71% and from 95.18 to 107.13%, respectively. The method was applied in studying the pharmacokinetics of lamotrigine administered orally to rabbits. This reliable micro-method would have application in pharmacokinetic studies of lamotrigine where only small sample sizes are available, e.g. paediatric patients.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dogs</subject><subject>Drug Stability</subject><subject>Evaluation Studies as Topic</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Lamotrigine</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pharmacokinetic studies</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasma</subject><subject>Rabbits</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><subject>Triazines - blood</subject><subject>Triazines - pharmacokinetics</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkEtrGzEQgEVpSZ20PyGgQynNYVvt6mWdSgh9QaCHptCbGM-OYpXdlSPJhf77KrHxtacZZr558DF22Yv3vejNhx_Cyr6zYq3fOXslxCBVJ5-xVb-2shuM-vWcrU7IS3Zeym8hhO6dOmNnzmjTG71id9c8wy6OfIoP-xZwm9MMNd236jYin6lu08hDyrxuiY9UKc9xgRrTwlPgE8yp5ngfF-Jx4bsJygyv2IsAU6HXx3jBfn7-dHfztbv9_uXbzfVth3LtageCrNAYNhZNy4xBi8rhIEiPthUk4GCDGZQ0qLQAUEpLxCFQ0Ao2QV6wt4e9u5we9lSqn2NBmiZYKO2Lt845ZQfTQH0AMadSMgW_y3GG_Nf3wj_a9E82_aMq76x_sullm7s8HthvZhpPU0d9rf_m2IeCMIUMC8ZywtqSQa5Vwz4eMGoy_kTKvmCkBWmMmbD6McX_PPIPxd6SDQ</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Matar, K.M</creator><creator>Nicholls, P.J</creator><creator>Bawazir, S.A</creator><creator>Al-Hassan, M.I</creator><creator>Tekle, A</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980701</creationdate><title>A rapid liquid chromatographic method for the determination of lamotrigine in plasma</title><author>Matar, K.M ; Nicholls, P.J ; Bawazir, S.A ; Al-Hassan, M.I ; Tekle, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-a0e705cfb7c6e7066c7c49c20e5d7e703ac27f62436c450aa4453cc2fef54abf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Dogs</topic><topic>Drug Stability</topic><topic>Evaluation Studies as Topic</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Lamotrigine</topic><topic>Liquid chromatography</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Pharmacokinetic studies</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasma</topic><topic>Rabbits</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><topic>Triazines - blood</topic><topic>Triazines - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matar, K.M</creatorcontrib><creatorcontrib>Nicholls, P.J</creatorcontrib><creatorcontrib>Bawazir, S.A</creatorcontrib><creatorcontrib>Al-Hassan, M.I</creatorcontrib><creatorcontrib>Tekle, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matar, K.M</au><au>Nicholls, P.J</au><au>Bawazir, S.A</au><au>Al-Hassan, M.I</au><au>Tekle, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid liquid chromatographic method for the determination of lamotrigine in plasma</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>17</volume><issue>3</issue><spage>525</spage><epage>531</epage><pages>525-531</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A rapid, sensitive and simple high-performance liquid chromatographic (HPLC) method for the determination of lamotrigine in plasma is described. The drug was extracted from 100 μl of plasma with chloroform:isopropanol (95:5% v/v) in the presence of 100 μl of phosphate buffer (10 mM). The extract was evaporated and the residue was reconstituted with mobile phase and injected onto the HPLC system. The drug and the internal standard (chloramphenicol) were eluted from a Symmetry C
18 stainless steel column at ambient temperature with a mobile phase consisting of 0.01 M potassium phosphate–acetonitrile–methanol (70:20:10% v/v/v), adjusted to pH 6.7, at a flow rate of 1.3 ml min
−1 and the detector was monitored at 214 nm. Quantitation was achieved by measurement of the peak-area ratio of the drug to the internal standard and the lower limit of detection for lamotrigine in plasma was 20 ng ml
−1. The intraday precision ranged from 3.34 to 6.12% coefficient of variation (CV) and the interday precision ranged from 2.15 to 8.34% CV. The absolute and relative recoveries of lamotrigine ranged from 86.93 to 90.71% and from 95.18 to 107.13%, respectively. The method was applied in studying the pharmacokinetics of lamotrigine administered orally to rabbits. This reliable micro-method would have application in pharmacokinetic studies of lamotrigine where only small sample sizes are available, e.g. paediatric patients.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9656165</pmid><doi>10.1016/S0731-7085(97)00234-3</doi><tpages>7</tpages></addata></record> |
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subjects | Analysis Animals Biological and medical sciences Chromatography, High Pressure Liquid - methods Dogs Drug Stability Evaluation Studies as Topic General pharmacology Humans Lamotrigine Liquid chromatography Male Medical sciences Pharmacokinetic studies Pharmacology. Drug treatments Plasma Rabbits Reproducibility of Results Sensitivity and Specificity Time Factors Triazines - blood Triazines - pharmacokinetics |
title | A rapid liquid chromatographic method for the determination of lamotrigine in plasma |
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